ArticleMolecular and cellular biochemistry2014
Lysine suppresses protein degradation through autophagic-lysosomal system in C2C12 myotubes.
Article in Molecular and cellular biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Dietary coated sodium selenite enhances muscle growth associated with mitochondrial function in sub-adult grass carp (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Differential regulation of autophagy and metabolic pathways by dietary methionine and lysine deficiency in goats.BMC veterinary research · 2026Article
- Analysis of the Stimulative Effect of Arginine on Translation Initiation of Protein Synthesis in Skeletal Muscle.Nutrients · 2025Article
- Role of ultrasonography in assessing respiratory muscle loss: insights from a cross-sectional study on neurological patients with long-term bed rest with and without tracheostomy.BMC pulmonary medicine · 2025Article
- Autophagy: Are Amino Acid Signals Dependent on the mTORC1 Pathway or Independent?Current issues in molecular biology · 2024Review
- Dietary Crude Protein and Lysine Levels Affect Meat Quality and Myofiber Characteristic of Slow-Growing Chicken.Animals : an open access journal from MDPI · 2024Article
- mTORC1 and BMP-Smad1/5 regulation of serum-stimulated myotube hypertrophy: a role for autophagy.American journal of physiology. Cell physiology · 2024Article
- Assessing Gluten-Free Soy Bread Quality and Amino Acid Content.Foods (Basel, Switzerland) · 2023Article
- The role of chicken eggs in modulating sarcopenic obesity and gut microbiota inFrontiers in microbiology · 2023Article
- Effects of the SLC38A2-mTOR Pathway Involved in Regulating the Different Compositions of Dietary Essential Amino Acids-Lysine and Methionine on Growth and Muscle Quality in Rabbits.Animals : an open access journal from MDPI · 2022Article
- Molecular and biochemical regulation of skeletal muscle metabolism.Journal of animal science · 2022Article
- Is the Brain Undernourished in Alzheimer's Disease?Nutrients · 2022Article
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- mTORC1 Mediates Lysine-Induced Satellite Cell Activation to Promote Skeletal Muscle Growth.Cells · 2019Article
- L-lysine protects C2C12 myotubes and 3T3-L1 adipocytes against high glucose damages and stresses.PloS one · 2019Article
- Age-related changes in skeletal muscle: changes to life-style as a therapy.Biogerontology · 2018Review
- α-Ketoglutarate prevents skeletal muscle protein degradation and muscle atrophy through PHD3/ADRB2 pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- A Systems Biology Approach Using Transcriptomic Data Reveals Genes and Pathways in Porcine Skeletal Muscle Affected by Dietary Lysine.International journal of molecular sciences · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Muscle mass is determined between protein synthesis and protein degradation. Reduction of muscle mass leads to bedridden condition and attenuation of resistance to diseases. Moreover, bedridden condition leads to additional muscle loss due to disuse muscle atrophy. In our previous study (Sato et al. 2013), we showed that administered lysine (Lys), one of essential amino acid, suppressed protein degradation in skeletal muscle. In this study, we investigated that the mechanism of the suppressive effects of Lys on skeletal muscle proteolysis in C2C12 cell line. C2C12 myotubes were incubated in the serum-free medium containing 10 mM Lys or 20 mM Lys, and myofibrillar protein degradation was determined by the rates of 3-methylhistidine (MeHis) release from the cells. The mammalian target of rapamycin (mTOR) activity from the phosphorylation levels of p70-ribosormal protein S6 kinase 1 and eIF4E-binding protein 1 and the autophagic-lysosomal system activity from the ratio of LC3-II/I in C2C12 myotubes stimulated by 10 mM Lys for 0-3 h were measured. The rates of MeHis release were markedly reduced by addition of Lys. The autophagic-lysosomal system activity was inhibited upon 30 min of Lys supplementation. The activity of mTOR was significantly increased upon 30 min of Lys supplementation. The suppressive effect of Lys on the proteolysis by the autophagic-lysosomal system was maintained partially when mTOR activity was inhibited by 100 nM rapamycin, suggesting that some regulator other than mTOR signaling, for example, Akt, might also suppress the autophagic-lysosomal system. From these results, we suggested that Lys suppressed the activity of the autophagic-lysosomal system in part through activation of mTOR and reduced myofibrillar protein degradation in C2C12 myotubes.
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