Evidence map›Paper›PMID 24531797›Full record

ArticleNature biotechnology2014

Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice.

Shrivats Mohan Iyer, Kate L Montgomery, Chris Towne, Soo Yeun Lee, Charu Ramakrishnan, Karl Deisseroth, Scott L Delp

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
114citing papers in PubMed, 2 pooled it
10.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

114 citing papers in PubMed, 2 syntheses or guidelines pooled it, 219 citations in OpenAlex.

  1. Pooled it
  2. Optogenetic control of the enteric nervous system and gastrointestinal transit.Expert review of gastroenterology & hepatology · 2019
    Pooled it
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Hes5Molecular brain · 2025
    Article
  12. Article
  13. Sensing a rainbow of colors: algal photoreceptors.Frontiers in plant science · 2025
    Review
  14. Gene therapy for chronic pain management.Cell reports. Medicine · 2024
    Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Optogenetic and Chemogenic Control of Pain Signaling: Molecular Markers.International journal of molecular sciences · 2023
    Review

54 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Shrivats Mohan Iyer1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2].
Kate L Montgomery1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2].
Chris TowneDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Soo Yeun LeeDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Charu RamakrishnanDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Karl Deisseroth1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.
Scott L Delp1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] Department of Mechanical Engineering, Stanford University, Stanford, California, USA.
Stanford University · USHoward Hughes Medical Institute · US

Funding

Optogenetic inhibition of motor neuron and muscle activity in vivoR01NS080954 · NINDS · STANFORD UNIVERSITY · PI DELP, SCOTT L · 2012 to 2015
$1.3M
Howard Hughes Medical InstituteNINDS NIH HHS R01 NS080954NINDS NIH HHS R01-NS080954
6 · The paper itself

Abstract

Primary nociceptors are the first neurons involved in the complex processing system that regulates normal and pathological pain. Because of constraints on pharmacological and electrical stimulation, noninvasive excitation and inhibition of these neurons in freely moving nontransgenic animals has not been possible. Here we use an optogenetic strategy to bidirectionally control nociceptors of nontransgenic mice. Intrasciatic nerve injection of adeno-associated viruses encoding an excitatory opsin enabled light-inducible stimulation of acute pain, place aversion and optogenetically mediated reductions in withdrawal thresholds to mechanical and thermal stimuli. In contrast, viral delivery of an inhibitory opsin enabled light-inducible inhibition of acute pain perception, and reversed mechanical allodynia and thermal hyperalgesia in a model of neuropathic pain. Light was delivered transdermally, allowing these behaviors to be induced in freely moving animals. This approach may have utility in basic and translational pain research, and enable rapid drug screening and testing of newly engineered opsins.

Indexed as

Disease Models, AnimalAnimalsBehavior, AnimalDependovirusDrug Delivery SystemsFemaleGenetic EngineeringGenetic TherapyInjectionsLightMiceMice, Inbred C57BLNociceptorsOpsinsOptogeneticsPainOpsins

Identifiers

PMID24531797
PMCPMC3988230
OpenAlexW2025645467

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.