ArticleG3 (Bethesda, Md.)2014
A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila.
Article in G3 (Bethesda, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A second genetic screen for gurken mRNA mislocalisation uncovers novel phenotypes of piRNA pathway mutants in Drosophila.Biology open · 2025Article
- Whole transcriptome screening for novel genes involved in meiosis and fertility in Drosophila melanogaster.Scientific reports · 2024Article
- Progress of CRISPR-Cas13 Mediated Live-Cell RNA Imaging and Detection of RNA-Protein Interactions.Frontiers in cell and developmental biology · 2022Review
- Live imaging and quantitation of nascent transcription using the MS2/MCP system in theSTAR protocols · 2021Article
- Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation.The Journal of cell biology · 2020Article
- Maelstrom Represses Canonical Polymerase II Transcription within Bi-directional piRNA Clusters in Drosophila melanogaster.Molecular cell · 2019Article
- Review
- Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.Chemical Society reviews · 2017Review
- The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition.Genes & development · 2016Article
- Fixed and live visualization of RNAs in Drosophila oocytes and embryos.Methods (San Diego, Calif.) · 2016Review
- The Krüppel-Like Factor Dar1 Determines Multipolar Neuron Morphology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- mRNA localization in the Drosophila germline.RNA biology · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We have screened chromosome arm 3L for ethyl methanesulfonate-induced mutations that disrupt localization of fluorescently labeled gurken (grk) messenger (m)RNA, whose transport along microtubules establishes both major body axes of the developing Drosophila oocyte. Rapid identification of causative mutations by single-nucleotide polymorphism recombinational mapping and whole-genomic sequencing allowed us to define nine complementation groups affecting grk mRNA localization and other aspects of oogenesis, including alleles of elg1, scaf6, quemao, nudE, Tsc2/gigas, rasp, and Chd5/Wrb, and several null alleles of the armitage Piwi-pathway gene. Analysis of a newly induced kinesin light chain allele shows that kinesin motor activity is required for both efficient grk mRNA localization and oocyte centrosome integrity. We also show that initiation of the dorsoanterior localization of grk mRNA precedes centrosome localization, suggesting that microtubule self-organization contributes to breaking axial symmetry to generate a unique dorsoventral axis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.