ArticleAnnals of biomedical engineering2014
Scaffold-free tissue engineering: organization of the tissue cytoskeleton and its effects on tissue shape.
Article in Annals of biomedical engineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Advances in 3D Bioprinting for Scaffold-Based and Scaffold-Free Tissue Engineering and Regenerative Medicine.Gels (Basel, Switzerland) · 2026Review
- From microtissues to macro solutions - The future of scalable and automated cartilage tissue engineering.Journal of orthopaedic translation · 2026Review
- Article
- A platform for Bioengineering Tissue Membranes from cell spheroids.Materials today. Bio · 2025Article
- Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox.Journal of biological engineering · 2024Article
- Spheroid-Based Tissue Engineering Strategies for Regeneration of the Intervertebral Disc.International journal of molecular sciences · 2022Review
- Mechanical properties of cell sheets and spheroids: the link between single cells and complex tissues.Biophysical reviews · 2021Review
- Vascular Tissue Engineering Using Scaffold-Free Prevascular Endothelial-Fibroblast Constructs.BioResearch open access · 2019Article
- Dental Pulp Stem Cell-Derived, Scaffold-Free Constructs for Bone Regeneration.International journal of molecular sciences · 2018Article
- Article
- Numerical Simulation of the Behavior of Toroidal and Spheroidal Multicellular Aggregates in Microfluidic Devices with Microwell and U-Shaped Barrier.Micromachines · 2017Article
- 3D Bioprinting for Vascularized Tissue Fabrication.Annals of biomedical engineering · 2017Review
- Article
- Laser-based technique for controlled damage of mesenchymal cell spheroids: a first step in studying reparation in vitro.Biology open · 2016Article
- Connexin-Based Therapeutics and Tissue Engineering Approaches to the Amelioration of Chronic Pancreatitis and Type I Diabetes: Construction and Characterization of a Novel Prevascularized Bioartificial Pancreas.Journal of diabetes research · 2016Article
- Implanted scaffold-free prevascularized constructs promote tissue repair.Annals of plastic surgery · 2015Article
- Self-assembly of prevascular tissues from endothelial and fibroblast cells under scaffold-free, nonadherent conditions.Tissue engineering. Part A · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Work described herein characterizes tissues formed using scaffold-free, non-adherent systems and investigates their utility in modular approaches to tissue engineering. Immunofluorescence analysis revealed that all tissues formed using scaffold-free, non-adherent systems organize tissue cortical cytoskeletons that appear to be under tension. Tension in these tissues was also evident when modules (spheroids) were used to generate larger tissues. Real-time analysis of spheroid fusion in unconstrained systems illustrated modular motion that is compatible with alterations in tensions, due to the process of disassembly/reassembly of the cortical cytoskeletons required for module fusion. Additionally, tissues generated from modules placed within constrained linear molds, which restrict modular motion, deformed upon release from molds. That tissue deformation is due in full or in part to imbalanced cortical actin cytoskeleton tensions resulting from the constraints imposed by mold systems is suggested from our finding that treatment of forming tissues with Y-27632, a selective inhibitor of ROCK phosphorylation, reduced tissue deformation. Our studies suggest that the deformation of scaffold-free tissues due to tensions mediated via the tissue cortical cytoskeleton represents a major and underappreciated challenge to modular tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.