ArticleNature structural & molecular biology2014
Structural basis for -10 promoter element melting by environmentally induced sigma factors.
Article in Nature structural & molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 87 citations in OpenAlex.
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- Single-stranded DNA drives σ subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations.Nucleic acids research · 2025Article
- Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σJournal of computer-aided molecular design · 2024Article
- Design of a tunable bacterial gene expression system using engineered σ factors.Applied and environmental microbiology · 2024Article
- Towards a rational approach to promoter engineering: understanding the complexity of transcription initiation in prokaryotes.FEMS microbiology reviews · 2024Review
- σCommunications biology · 2024Article
- Structure of the transcription open complex of distinct σNature communications · 2023Article
- Structural Insight into the Mechanism of σ32-Mediated Transcription Initiation of Bacterial RNA Polymerase.Biomolecules · 2023Article
- A general mechanism for transcription bubble nucleation in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Alteration of the -35 and -10 sequences and deletion the upstream sequence of the -35 region of the promoter A1 of the phage T7 in dsDNA confirm the contribution of non-specific interactions withFrontiers in molecular biosciences · 2023Article
- Expanding the genetic toolkit helps dissect a global stress response in the early-branching speciesProceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Burkholderia cenocepacia iron starvation σ factor, OrbS, possesses an on-board iron sensor.Nucleic acids research · 2022Article
- The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA.Environmental microbiology · 2022Article
- Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Overlapping SigH and SigE sigma factor regulons inFrontiers in microbiology · 2022Article
- The anti-sigma factor MucA is required for viability in Pseudomonas aeruginosa.Molecular microbiology · 2021Article
- Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.International journal of molecular sciences · 2021Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli σ(E), which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.