ArticleEnvironmental health perspectives2014
Effects of arsenic on osteoblast differentiation in vitro and on bone mineral density and microstructure in rats.
Article in Environmental health perspectives, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Airborne contaminant exposure and bone development: a systematic review and meta-analysis.Toxicological sciences : an official journal of the Society of Toxicology · 2026Pooled it
- Folic Acid and Creatine as Therapeutic Approaches to Lower Blood Arsenic: A Randomized Controlled Trial.Environmental health perspectives · 2015Trial
- Metallic Contaminants and Osteoporosis: a Review of the Gut-Bone Axis.Biological trace element research · 2026Review
- Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis.Archives of toxicology · 2024Review
- Association between urinary arsenic species and vitamin D deficiency: a cross-sectional study in Chinese pregnant women.Frontiers in public health · 2024Article
- Effects of Co-exposure to Fluoride and Arsenic on TRAF-6 Signaling and NF-κB Pathway of Bone Metabolism.Biological trace element research · 2023Article
- Associations between Plasma Folate and Vitamin BNutrients · 2022Observational
- The osteogenic niche-targeted arsenic nanoparticles prevent colonization of disseminated breast tumor cells in the bone.Acta pharmaceutica Sinica. B · 2022Article
- Lead exposure and indices of height and weight in Uruguayan urban school children, considering co-exposure to cadmium and arsenic, sex, iron status and dairy intake.Environmental research · 2021Article
- Arsenic, cadmium, and selenium exposures and bone mineral density-related endpoints: The HORTEGA study.Free radical biology & medicine · 2021Article
- UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging.PeerJ · 2021Article
- Alcohol Consumption Moderated the Association Between Levels of High Blood Lead or Total Urinary Arsenic and Bone Loss.Frontiers in endocrinology · 2021Article
- Factors Affecting the Aluminum, Arsenic, Cadmium and Lead Concentrations in the Knee Joint Structures.Frontiers in public health · 2021Article
- Molecular and cellular mechanisms linking air pollution and bone damage.Environmental research · 2020Review
- Nicotine exposure during pregnancy programs osteopenia in male offspring ratsFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- First trimester maternal exposures to endocrine disrupting chemicals and metals and fetal size in the Michigan Mother-Infant Pairs study.Journal of developmental origins of health and disease · 2019Article
- A Review of Metal Exposure and Its Effects on Bone Health.Journal of toxicology · 2018Review
- In Vivo Exposure to Inorganic Arsenic Alters Differentiation-Specific Gene Expression of Adipose-Derived Mesenchymal Stem/Stromal Cells in C57BL/6J Mouse Model.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- Environmental Factors Impacting Bone-Relevant Chemokines.Frontiers in endocrinology · 2017Article
- Tungsten Promotes Sex-Specific Adipogenesis in the Bone by Altering Differentiation of Bone Marrow-Resident Mesenchymal Stromal Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundArsenic is a ubiquitous toxic element and is known to contaminate drinking water in many countries. Several epidemiological studies have shown that arsenic exposure augments the risk of bone disorders. However, the detailed effect and mechanism of inorganic arsenic on osteoblast differentiation of bone marrow stromal cells and bone loss still remain unclear.
objectivesWe investigated the effects and mechanism of arsenic on osteoblast differentiation in vitro and evaluated bone mineral density (BMD) and bone microstructure in rats at doses relevant to human exposure from drinking water.
methodsWe used a cell model of rat primary bone marrow stromal cells (BMSCs) and a rat model of long-term exposure with arsenic-contaminated drinking water, and determined bone microstructure and BMD in rats by microcomputed tomography (μCT).
resultsWe observed significant attenuation of osteoblast differentiation after exposure of BMSCs to arsenic trioxide (0.5 or 1 μM). After arsenic treatment during differentiation, expression of runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), and osteocalcin in BMSCs was inhibited and phosphorylation of enhanced extracellular signal-regulated kinase (ERK) was increased. These altered differentiation-related molecules could be reversed by the ERK inhibitor PD98059. Exposure of rats to arsenic trioxide (0.05 or 0.5 ppm) in drinking water for 12 weeks altered BMD and microstructure, decreased Runx2 expression, and increased ERK phosphorylation in bones. In BMSCs isolated from arsenic-treated rats, osteoblast differentiation was inhibited.
conclusionsOur results suggest that arsenic is capable of inhibiting osteoblast differentiation of BMSCs via an ERK-dependent signaling pathway and thus increasing bone loss.
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