ArticleAtherosclerosis2014
Effect of low extracellular pH on NF-κB activation in macrophages.
Article in Atherosclerosis, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- Function and contribution of two putativeInfection and immunity · 2024Article
- An acidic microenvironment in Tuberculosis increases extracellular matrix degradation by regulating macrophage inflammatory responses.PLoS pathogens · 2023Article
- Apoptotic body-inspired nanoparticles target macrophages at sites of inflammation to support an anti-inflammatory phenotype shift.International journal of pharmaceutics · 2022Article
- Betulin Sulfonamides as Carbonic Anhydrase Inhibitors and Anticancer Agents in Breast Cancer Cells.International journal of molecular sciences · 2021Article
- Differential Effect of Extracellular Acidic Environment on IL-1β Released from Human and Mouse Phagocytes.International journal of molecular sciences · 2020Article
- Functional regeneration of tissue engineered skeletal muscle in vitro is dependent on the inclusion of basement membrane proteins.Cytoskeleton (Hoboken, N.J.) · 2019Article
- Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells.Molecular medicine reports · 2019Article
- Acidosis exacerbates in vivo IL-1-dependent inflammatory responses and neutrophil recruitment during pulmonary Pseudomonas aeruginosa infection.American journal of physiology. Lung cellular and molecular physiology · 2018Article
- Unravelling the Interplay between Extracellular Acidosis and Immune Cells.Mediators of inflammation · 2018Review
- Targeting breast cancer cells by MRS1477, a positive allosteric modulator of TRPV1 channels.PloS one · 2017Article
- The function of cathepsins B, D, and X in atherosclerosis.American journal of cardiovascular disease · 2016Article
- Metabolic Connection of Inflammatory Pain: Pivotal Role of a Pyruvate Dehydrogenase Kinase-Pyruvate Dehydrogenase-Lactic Acid Axis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Accelerated vascular disease in systemic lupus erythematosus: role of macrophage.Clinical immunology (Orlando, Fla.) · 2015Review
- Acidification of the intimal fluid: the perfect storm for atherogenesis.Journal of lipid research · 2015Review
- Mesenchymal stem cells alleviate atherosclerosis by elevating number and function of CD4(+)CD25 (+)FOXP3 (+) regulatory T-cells and inhibiting macrophage foam cell formation.Molecular and cellular biochemistry · 2015Article
- Correlation between mimecan expression and coronary artery stenosis in patients with coronary heart disease.International journal of clinical and experimental medicine · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
objectiveMany diseases, including atherosclerosis, involve chronic inflammation. The master transcription factor for inflammation is NF-κB. Inflammatory sites have a low extracellular pH. Our objective was to demonstrate the effect of pH on NF-κB activation and cytokine secretion.
methodsMouse J774 macrophages or human THP-1 or monocyte-derived macrophages were incubated at pH 7.0-7.4 and inflammatory cytokine secretion and NF-κB activity were measured.
resultsA pH of 7.0 greatly decreased pro-inflammatory cytokine secretion (TNF or IL-6) by J774 macrophages, but not THP-1 or human monocyte-derived macrophages. Upon stimulation of mouse macrophages, the levels of IκBα, which inhibits NF-κB, fell but low pH prevented its later increase, which normally restores the baseline activity of NF-κB, even though the levels of mRNA for IκBα were increased. pH 7.0 greatly increased and prolonged NF-κB binding to its consensus promoter sequence, especially the anti-inflammatory p50:p50 homodimers. Human p50 was overexpressed using adenovirus in THP-1 macrophages and monocyte-derived macrophages to see if it would confer pH sensitivity to NF-κB activity in human cells. Overexpression of p50 increased p50:p50 DNA-binding and in THP-1 macrophages inhibited considerably TNF and IL-6 secretion, but there was still no effect of pH on p50:p50 DNA binding or cytokine secretion.
conclusionA modest decrease in pH can sometimes have marked effects on NF-κB activation and cytokine secretion and might be one reason to explain why mice normally develop less atherosclerosis than do humans.
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