ArticleMethods in enzymology2014
Optimal protocol for the differentiation and metabolic analysis of human adipose stromal cells.
Article in Methods in enzymology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Postbiotic Metabolites of Proanthocyanidins Reduce Adipogenesis In Vitro by Suppressing De Novo Lipogenesis.Molecules (Basel, Switzerland) · 2026Article
- Adipose tissue as a living drug: Stromal vascular fraction and adipose tissue-derived stem cells in regenerative medicine.World journal of stem cells · 2025Review
- Adipocyte-derived factors induce adherent to suspension transition in breast and pancreatic cancer cells through lipid metabolic alteration.Scientific reports · 2025Article
- Vitamin DNutrients · 2025Article
- Investigating the role of adipose tissue in mobility and aging: design and methods of the Adipose Tissue ancillary to the Study of Muscle, Mobility, and Aging (SOMMA-AT).The journals of gerontology. Series A, Biological sciences and medical sciences · 2025Article
- Adipogenic Differentiation Assay for the Study of Insulin Signaling.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Transcription factor PATZ1 promotes adipogenesis by controlling promoter regulatory loci of adipogenic factors.Nature communications · 2024Article
- Dissecting human adipose tissue heterogeneity using single-cell omics technologies.Stem cell research & therapy · 2024Review
- AGPAT3 deficiency impairs adipocyte differentiation and leads to a lean phenotype in mice.American journal of physiology. Endocrinology and metabolism · 2024Article
- Adipogenic differentiation of hematopoietic lineage cells isolated from adipose tissue of humans.bioRxiv : the preprint server for biology · 2024Article
- Repurposing lansoprazole to alleviate metabolic syndromeActa pharmaceutica Sinica. B · 2024Article
- Reconstructed Human Skin with Hypodermis Shows Essential Role of Adipose Tissue in Skin Metabolism.Tissue engineering and regenerative medicine · 2024Article
- An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis.Adipocyte · 2023Article
- Standardized In Vitro Models of Human Adipose Tissue Reveal Metabolic Flexibility in Brown Adipocyte Thermogenesis.Endocrinology · 2023Article
- Impaired Adipocyte SLC7A10 Promotes Lipid Storage in Association With Insulin Resistance and Altered BCAA Metabolism.The Journal of clinical endocrinology and metabolism · 2023Article
- A Rare Olive Compound Oleacein Improves Lipid and Glucose Metabolism, and Inflammatory Functions: A Comprehensive Whole-Genome Transcriptomics Analysis in Adipocytes Differentiated from Healthy and Diabetic Adipose Stem Cells.International journal of molecular sciences · 2023Article
- A Liquid Biopsy-Based Approach to Isolate and Characterize Adipose Tissue-Derived Extracellular Vesicles from Blood.ACS nano · 2023Article
- Bovine Fibroblast-Derived Extracellular Matrix Promotes the Growth and Preserves the Stemness of Bovine Stromal Cells during In Vitro Expansion.Journal of functional biomaterials · 2023Article
- Measuring the Rate of Lipolysis in Ex vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro.Journal of visualized experiments : JoVE · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
Abstract
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be isolated from distinct adipose depots and cultured in vitro, have clear translational relevance. We describe the methods to isolate, culture, and differentiate human ASCs to adipocytes that respond to physiologically relevant hormones, such as insulin and β-adrenergic agonists. We also describe methods for assaying hormonal effects on glucose transport and lipolysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.