ArticleBMC genomics2014
Comprehensive identification of novel proteins and N-glycosylation sites in royal jelly.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
- Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions inJournal of Zhejiang University. Science. B · 2026Article
- A queen's tale: An experimental palaeoproteomic study of a honey bee queen cell specimen from Natural History Museum Denmark.Open research Europe · 2024Article
- Article
- Identification of N-linked Glycoproteins in Silkworm Serum Using Con A Lectin Affinity Chromatography and Mass Spectrometry.Journal of insect science (Online) · 2021Article
- Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples.Molecules (Basel, Switzerland) · 2021Article
- Royal Jelly-A Traditional and Natural Remedy for Postmenopausal Symptoms and Aging-Related Pathologies.Molecules (Basel, Switzerland) · 2020Review
- New Insights into the Biological and Pharmaceutical Properties of Royal Jelly.International journal of molecular sciences · 2020Review
- The Emerging Proteomic Research Facilitates in-Depth Understanding of the Biology of Honeybees.International journal of molecular sciences · 2019Review
- The rise and fall of major royal jelly proteins during a honeybee (Ecology and evolution · 2019Article
- In-depth Proteome of the Hypopharyngeal Glands of Honeybee Workers Reveals Highly Activated Protein and Energy Metabolism in Priming the Secretion of Royal Jelly.Molecular & cellular proteomics : MCP · 2019Article
- Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins.Molecular & cellular proteomics : MCP · 2018Article
- Transgenic silkworms secrete the recombinant glycosylated MRJP1 protein of Chinese honeybee, Apis cerana cerana.Transgenic research · 2017Article
- Comparative analyses of the major royal jelly protein gene cluster in three Apis species with long amplicon sequencing.DNA research : an international journal for rapid publication of reports on genes and genomes · 2017Article
- Article
- Article
- The transcriptomic and evolutionary signature of social interactions regulating honey bee caste development.Ecology and evolution · 2015Article
- MRJP1-containing glycoproteins isolated from honey, a novel antibacterial drug candidate with broad spectrum activity against multi-drug resistant clinical isolates.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRoyal jelly (RJ) is a proteinaceous secretion produced from the hypopharyngeal and mandibular glands of nurse bees. It plays vital roles in honeybee biology and in the improvement of human health. However, some proteins remain unknown in RJ, and mapping N-glycosylation modification sites on RJ proteins demands further investigation. We used two different liquid chromatography-tandem mass spectrometry techniques, complementary N-glycopeptide enrichment strategies, and bioinformatic approaches to gain a better understanding of novel and glycosylated proteins in RJ.
resultsA total of 25 N-glycosylated proteins, carrying 53 N-glycosylation sites, were identified in RJ proteins, of which 42 N-linked glycosylation sites were mapped as novel on RJ proteins. Most of the glycosylated proteins were related to metabolic activities and health improvement. The 13 newly identified proteins were also mainly associated with metabolic processes and health improvement activities.
conclusionOur in-depth, large-scale mapping of novel glycosylation sites represents a crucial step toward systematically revealing the functionality of N-glycosylated RJ proteins, and is potentially useful for producing a protein with desirable pharmacokinetic and biological activity using a genetic engineering approach. The newly-identified proteins significantly extend the proteome coverage of RJ. These findings contribute vital and new knowledge to our understanding of the innate biochemical nature of RJ at both the proteome and glycoproteome levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.