Evidence map›Paper›PMID 24528743›Full record

ArticleCarbohydrate polymers2014

Augmenting protein release from layer-by-layer functionalized agarose hydrogels.

Daniel Lynam, Chelsea Peterson, Ryan Maloney, Dena Shahriari, Alexa Garrison, Sara Saleh, Sumit Mehrotra, Christina Chan, Jeff Sakamoto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Carbohydrate polymers, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Minimally Invasive and Regenerative Therapeutics.Advanced materials (Deerfield Beach, Fla.) · 2019
    Review
  6. Thermoresponsive Gels.Gels (Basel, Switzerland) · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Daniel LynamDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA.
Chelsea PetersonDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA.
Ryan MaloneyDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA.
Dena ShahriariDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA.
Alexa GarrisonDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA.
Sara SalehDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA.
Sumit MehrotraDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA.
Christina ChanDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
Jeff SakamotoDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, USA. Electronic address: jsakamot@msu.edu.

Funding

Bioengineered Scaffolds for Spinal Cord InjuryR01EB014986 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SAKAMOTO, JEFFREY, TUSZYNSKI, MARK H. · 2012 to 2016
$2.4M
NIBIB NIH HHS R01 EB014986NIBIB NIH HHS R01EB014986
6 · The paper itself

Abstract

Recent work demonstrated the efficacy of combining layer-by-layer assembly with hydrogels to provide the controlled delivery of proteins for use in nerve repair scaffolds. In this work, we augmented the protein dose response by controlling and increasing the hydrogel internal surface area. Sucrose was added to agarose during gelation to homogenize the nanopore morphology, resulting in increased surface area per unit volume of hydrogel. The surface area of a range of compositions (1.5-5.0 wt% agarose and 0, 50 and 65 wt% sucrose) was measured. Gels were supercritically dried to preserve porosity enabling detailed pore morphology measurements using nitrogen adsorption and high resolution scanning electron microscopy. The resulting surface area, normalized by superficial gel volume, ranged between 6m(2)/cm(3)gel and 56 m(2)/cm(3)gel. Using the layer-by-layer process to load lysozyme, a neurotrophic factor analog, a relationship was observed between surface area and cumulative dose response ranging from 176 to 2556 μg/mL, which is in the range of clinical relevance for the delivery of growth factors. In this work, we demonstrated that the ability to control porosity is key in tuning drug delivery dose response from layer-by-layer modified hydrogels.

Indexed as

HydrogelsParticle SizeProteinsSepharoseSurface PropertiesHydrogelsProteinsSepharoseControlled drug releaseHydrogelLayer-by-layerPore refinementSurface area

Identifiers

PMID24528743
PMCPMC3986118

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.