ArticleMolecular microbiology2014
Maf-dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar T3SS export.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Flagellar glycosylation with pseudaminic acids is widespread in the genus Clostridium.BMC microbiology · 2026Article
- The high efficiency protective effectiveness of a newly isolated myoviruses bacteriophage vB_AceP_PAc in protecting mice from Aeromonas caviae infection in mice.BMC microbiology · 2025Article
- Harnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras.Communications biology · 2024Article
- Stereoisomer-specific reprogramming of a bacterial flagellin sialyltransferase.The EMBO journal · 2023Article
- Heterogeneous glycosylation and methylation of the Aeromonas caviae flagellin.MicrobiologyOpen · 2022Article
- Azide-Functionalized Derivatives of the Virulence-Associated Sugar Pseudaminic Acid: Chiral Pool Synthesis and Labeling of Bacteria.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- The dental plaque biofilm matrix.Periodontology 2000 · 2021Review
- Glycosyltransferase Jhp0106 (PseE) contributes to flagellin maturation in Helicobacter pylori.Helicobacter · 2021Article
- Article
- Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity.Microorganisms · 2020Review
- Polar Flagella Glycosylation inFrontiers in microbiology · 2020Article
- Protein post-translational modifications in bacteria.Nature reviews. Microbiology · 2019Review
- Draft Whole-Genome Sequences of 10Microbiology resource announcements · 2019Article
- Nonulosonic acids contribute to the pathogenicity of the oral bacteriumInterface focus · 2019Review
- Identification ofFrontiers in microbiology · 2019Article
- Article
- Importance ofFrontiers in microbiology · 2017Article
- Flagellin glycosylation in Paenibacillus alvei CCM 2051T.Glycobiology · 2016Article
- The Aeromonas caviae AHA0618 gene modulates cell length and influences swimming and swarming motility.MicrobiologyOpen · 2015Article
- The polar and lateral flagella from Plesiomonas shigelloides are glycosylated with legionaminic acid.Frontiers in microbiology · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial swimming is mediated by rotation of a filament that is assembled via polymerization of flagellin monomers after secretion via a dedicated flagellar Type III secretion system. Several bacteria decorate their flagellin with sialic acid related sugars that is essential for motility. Aeromonas caviae is a model organism for this process as it contains a genetically simple glycosylation system and decorates its flagellin with pseudaminic acid (Pse). The link between flagellin glycosylation and export has yet to be fully determined. We examined the role of glycosylation in the export and assembly process in a strain lacking Maf1, a protein involved in the transfer of Pse onto flagellin at the later stages of the glycosylation pathway. Immunoblotting, established that glycosylation is not required for flagellin export but is essential for filament assembly since non-glycosylated flagellin is still secreted. Maf1 interacts directly with its flagellin substrate in vivo, even in the absence of pseudaminic acid. Flagellin glycosylation in a flagellin chaperone mutant (flaJ) indicated that glycosylation occurs in the cytoplasm before chaperone binding and protein secretion. Preferential chaperone binding to glycosylated flagellin revealed its crucial role, indicating that this system has evolved to favour secretion of the polymerization competent glycosylated form.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.