Evidence map›Paper›PMID 24526819›Full record

ArticleJournal of toxicologic pathology2013

Nuclear Morphometric Analysis of Leydig Cells of Male Pubertal Rats Exposed In Utero to Di(n-butyl) Phthalate.

Shin Wakui, Masaya Motohashi, Takemi Satoh, Masaru Shirai, Tomoko Mutou, Hiroyuki Takahashi, Michael F Wempe, Hitoshi Endou, Tomoo Inomata, Masao Asari

RetractedOpen access · bronzeAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of toxicologic pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Shin WakuiDepartment of Toxicology, Laboratory Animal Science, and Veterinary Anatomy, Azabu University School of Veterinary Medicine, 1-17-71 Chuo, Sagamihara, Kanagawa 252-5201, Japan.
Masaya MotohashiDepartment of Toxicology, Laboratory Animal Science, and Veterinary Anatomy, Azabu University School of Veterinary Medicine, 1-17-71 Chuo, Sagamihara, Kanagawa 252-5201, Japan.
Takemi SatohKokusan Co., Ltd., 7-8-16 Nishibori Sakura, Saitama 338-0832, Japan.
Masaru ShiraiDepartment of Toxicology, Laboratory Animal Science, and Veterinary Anatomy, Azabu University School of Veterinary Medicine, 1-17-71 Chuo, Sagamihara, Kanagawa 252-5201, Japan.
Tomoko MutouDrug Safety Testing Center, 25-1 Kuroiwa, Yoshimi Hiki, Saitama 335-0116, Japan.
Hiroyuki TakahashiDepartment of Pathology, The Jikei University School of Medicine, 3-35-8 Nishishimbashi, Minato, Tokyo 105-8461, Japan.
Michael F WempeSchool of Pharmacy, University of Colorado, Anschutz Medical Campus, 12850 East Montview Blvd., Aurora, CO 80045, USA.
Hitoshi EndouJ-Pharma Co., Ltd., 75-1 Turumi, Kanagawa 230-0046, Japan.
Tomoo InomataDepartment of Toxicology, Laboratory Animal Science, and Veterinary Anatomy, Azabu University School of Veterinary Medicine, 1-17-71 Chuo, Sagamihara, Kanagawa 252-5201, Japan.
Masao AsariDepartment of Toxicology, Laboratory Animal Science, and Veterinary Anatomy, Azabu University School of Veterinary Medicine, 1-17-71 Chuo, Sagamihara, Kanagawa 252-5201, Japan.
Azabu University · JPJikei University School of Medicine · JPUniversity of Colorado Anschutz Medical Campus · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We recently reported that prenatal rat exposure to di(n-butyl) phthalate (DBP) induced Leydig cell (LC) hyperplasia after nine weeks (wks) of age, yet the number of LCs was similar to that of the vehicle group until seven weeks. Nuclear pleomorphism of hyperplastic LCs is common and is considered to be continuous progressive degeneration. Thus, computer-assisted image cell nuclear analysis of LCs was performed on 5- and 7-wk-old Sprague-Dawley (SD) rats whose dams had been administered DBP (i.g.) at 100 mg/kg/day or vehicle (corn oil) on gestation day 12 to 21. The results of the 5-wk-old DBP group were similar to those of the vehicle group; LC nuclei of the 7-wk-old DBP group showed normal ploidy and similar amounts of DNA. However, the size, elongation and peripheral chromatin aggregation parameters were significantly higher, and the reticular chromatin distribution and isolated chromatin aggregation parameters were significantly lower compared with the vehicle group. The present study quantitatively demonstrated nuclear morphological alterations in rat LCs at 7 wks old (puberty) due to the prenatal DBP administration before apparent LC hyperplasia developed.

Indexed as

computer-assisted image cell nuclear analysisLeydig cellprenatal DBP exposureratstestis

Identifiers

PMID24526819
PMCPMC3921929
OpenAlexW1991154148

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.