ArticleThe Journal of infectious diseases2014
Increased neutrophil extracellular trap-mediated Staphylococcus aureus clearance through inhibition of nuclease activity by clindamycin and immunoglobulin.
Article in The Journal of infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- Prediction of virulence factors in bacterial hypothetical proteins.Archives of microbiology · 2025Article
- Broadening Horizons: Exploring the Cathepsin Family as Therapeutic Targets for Alzheimer's Disease.Aging and disease · 2024Review
- Injectable Platelet-Rich Fibrin as a Drug Carrier Increases the Antibacterial Susceptibility of Antibiotic-Clindamycin Phosphate.International journal of molecular sciences · 2022Article
- Molecular Prerequisites for Neutrophil Extracellular Trap Formation and Evasion Mechanisms ofFrontiers in immunology · 2022Review
- Pathogen-Derived Nucleases: An Effective Weapon for Escaping Extracellular Traps.Frontiers in immunology · 2022Review
- Review
- Staphylococcus aureus uses the ArlRS and MgrA cascade to regulate immune evasion during skin infection.Cell reports · 2021Article
- Influence of sub-inhibitory concentrations of antimicrobials on micrococcal nuclease and biofilm formation in Staphylococcus aureus.Scientific reports · 2021Article
- Review
- Correlations of Host and Bacterial Characteristics with Clinical Parameters and Survival inJournal of clinical medicine · 2021Article
- Invasive Group A Streptococcal Infections: Benefit of Clindamycin, Intravenous Immunoglobulins and Secondary Prophylaxis.Frontiers in pediatrics · 2021Review
- Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Peptidylarginine Deiminase 2 in Host Immunity: Current Insights and Perspectives.Frontiers in immunology · 2021Review
- Neutrophils exhibit an individual response to different oral bacterial biofilms.Journal of oral microbiology · 2020Article
- Host-Pathogen Interactions in Gram-Positive Bacterial Pneumonia.Clinical microbiology reviews · 2019Review
- High Nuclease Activity of Long PersistingFrontiers in immunology · 2019Article
- The Intracellular Cyclophilin PpiB Contributes to the Virulence of Staphylococcus aureus Independently of Its Peptidyl-ProlylInfection and immunity · 2018Article
- Cathepsins: Proteases that are vital for survival but can also be fatal.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2018Review
- Neutrophils and neutrophil extracellular traps in the liver and gastrointestinal system.Nature reviews. Gastroenterology & hepatology · 2018Review
- Targeting fundamental pathways to disrupt Staphylococcus aureus survival: clinical implications of recent discoveries.JCI insight · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Gram-positive human pathogen Staphylococcus aureus causes a variety of human diseases such as skin infections, pneumonia, and endocarditis. The micrococcal nuclease Nuc1 is one of the major S. aureus virulence factors and allows the bacterium to avoid neutrophil extracellular trap (NET)-mediated killing. We found that addition of the protein synthesis inhibitor clindamycin to S. aureus LAC cultures decreased nuc1 transcription and subsequently blunted nuclease activity in a molecular beacon-based fluorescence assay. We also observed reduced NET degradation through Nuc1 inhibition translating into increased NET-mediated clearance. Similarly, pooled human immunoglobulin specifically inhibited nuclease activity in a concentration-dependent manner. Inhibition of nuclease activity by clindamycin and immunoglobulin enhanced S. aureus clearance and should be considered in the treatment of S. aureus infections.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.