ArticleInvestigative ophthalmology & visual science2014
R9AP overexpression alters phototransduction kinetics in iCre75 mice.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Off-Target Retinal Pigment Epithelium (RPE) Expression of Cre in the Rhodopsin-iCre75 Mouse Line.Investigative ophthalmology & visual science · 2026Article
- Chromophore supply modulates cone function and survival in retinitis pigmentosa mouse models.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cone-Driven Retinal Responses Are Shaped by Rod But Not Cone HCN1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Pathogenesis of Eosinophilic Esophagitis: A Comprehensive Review of the Genetic and Molecular Aspects.International journal of molecular sciences · 2020Review
- Dephosphorylation by protein phosphatase 2A regulates visual pigment regeneration and the dark adaptation of mammalian photoreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Genetics of eosinophilic esophagitis.Mucosal immunology · 2017Review
- Transcriptome profiling of NIH3T3 cell lines expressing opsin and the P23H opsin mutant identifies candidate drugs for the treatment of retinitis pigmentosa.Pharmacological research · 2017Article
- CRALBP supports the mammalian retinal visual cycle and cone vision.The Journal of clinical investigation · 2015Article
- Effect of knocking down the insulin receptor on mouse rod responses.Scientific reports · 2015Article
- Chemistry and biology of the initial steps in vision: the Friedenwald lecture.Investigative ophthalmology & visual science · 2014Article
- DICER1 is essential for survival of postmitotic rod photoreceptor cells in mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Ex vivo ERG analysis of photoreceptors using an in vivo ERG system.Vision research · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeDetermine the impact of rod photoreceptor-specific expression of Cre recombinase on the kinetics of phototransduction in the mouse eye and identify changes in gene expression that underlie any observed phenotypic differences.
methodsTransretinal ERG and single-cell suction electrode recordings were used to measure the kinetics of phototransduction in a mouse line exhibiting rod photoreceptor-specific Cre recombinase expression, and the results were compared with those from control non-Cre-expressing littermates. Gene expression changes were evaluated using RNA sequencing transcriptome analysis. The pattern of expression of Rgs9bp was determined by mapping sequencing reads to the mouse genome and performing 3'-rapid amplification of cDNA ends (3'-RACE).
resultsExpression of the rod-specific iCre75 transgene was accompanied by accelerated phototransduction inactivation, likely due to overexpression of the Rgs9bp gene, which encodes the Rgs9 anchor protein (R9AP). R9AP upregulation stabilized the RGS9 GAP complex, altering phototransduction kinetics. 3'-Race identified an abundant, unexpected Rgs9bp-Prm1 fusion mRNA in Cre-expressing mouse retinas, which was determined to be derived from a second transgene present in the iCre75 line.
conclusionsHere we report the presence of a second, R9AP-expressing transgene in the iCre75 mouse line, leading to altered kinetics of phototransduction. These results highlight an important caveat that must be considered when utilizing this mouse line for rod photoreceptor-specific gene loss of function studies.
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