Evidence map›Paper›PMID 24526308›Full record

ArticleNeuro-oncology2014

Regulatory factor X1 is a new tumor suppressive transcription factor that acts via direct downregulation of CD44 in glioblastoma.

Chenzhuo Feng, Ying Zhang, Jinbo Yin, Jun Li, Roger Abounader, Zhiyi Zuo

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuro-oncology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 38 citations in OpenAlex.

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  20. Primary cilium and glioblastoma.Therapeutic advances in medical oncology · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Chenzhuo FengDepartment of Anesthesiology, University of Virginia, Charlottesville, VA, USA (C.F., J.Y., J.L., Z.Z.); Department of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA, USA (Y.Z., R.A.); Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China (J.Y.); Department of Anesthesiology, Fourth Affiliated Hospital, Harbin Medical University, Harbin, China (J.L.).
Ying ZhangDepartment of Anesthesiology, University of Virginia, Charlottesville, VA, USA (C.F., J.Y., J.L., Z.Z.); Department of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA, USA (Y.Z., R.A.); Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China (J.Y.); Department of Anesthesiology, Fourth Affiliated Hospital, Harbin Medical University, Harbin, China (J.L.).
Jinbo YinDepartment of Anesthesiology, University of Virginia, Charlottesville, VA, USA (C.F., J.Y., J.L., Z.Z.); Department of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA, USA (Y.Z., R.A.); Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China (J.Y.); Department of Anesthesiology, Fourth Affiliated Hospital, Harbin Medical University, Harbin, China (J.L.).
Jun LiDepartment of Anesthesiology, University of Virginia, Charlottesville, VA, USA (C.F., J.Y., J.L., Z.Z.); Department of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA, USA (Y.Z., R.A.); Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China (J.Y.); Department of Anesthesiology, Fourth Affiliated Hospital, Harbin Medical University, Harbin, China (J.L.).
Roger AbounaderDepartment of Anesthesiology, University of Virginia, Charlottesville, VA, USA (C.F., J.Y., J.L., Z.Z.); Department of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA, USA (Y.Z., R.A.); Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China (J.Y.); Department of Anesthesiology, Fourth Affiliated Hospital, Harbin Medical University, Harbin, China (J.L.).
Zhiyi ZuoDepartment of Anesthesiology, University of Virginia, Charlottesville, VA, USA (C.F., J.Y., J.L., Z.Z.); Department of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA, USA (Y.Z., R.A.); Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China (J.Y.); Department of Anesthesiology, Fourth Affiliated Hospital, Harbin Medical University, Harbin, China (J.L.).
Army Medical University · CNUniversity of Virginia · USXinqiao Hospital · CN

Funding

Receptor Tyrosine Kinase/PTEN Interactions in GliomasR01NS045209 · NINDS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI ABOUNADER, ROGER · 2003 to 2017
$4.1M
Volatile anesthetic modulation of glutamate transportersR01GM065211 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI ZUO, ZHIYI · 2003 to 2011
$2.6M
Molecular interactions and restoration strategies of PTEN and p53 in gliomasR01CA134843 · NCI · UNIVERSITY OF VIRGINIA · PI ABOUNADER, ROGER · 2010 to 2014
$1.5M
Volatile anesthetic effects on aging brainR01GM098308 · NIGMS · UNIVERSITY OF VIRGINIA · PI ZUO, ZHIYI · 2012 to 2015
$1.2M
NCI NIH HHS R01 CA134843NIGMS NIH HHS R01 GM065211NIGMS NIH HHS R01 GM098308NINDS NIH HHS R01 NS045209
6 · The paper itself

Abstract

backgroundThe biological functions of regulatory factor (RF)X1, a transcription factor, are not known. Since the RFX1 gene is often epigenetically silenced and clusters of differentiation (CD)44 proteins that regulate cancer cell biology are increased in human glioblastomas, we designed this study to determine whether RFX1 could regulate CD44 expression in glioblastoma.

methodsRegulatory factor X1 was overexpressed in 4 human glioblastoma cell lines. CD44 expression and cell proliferation, apoptosis, and invasion were assayed under in vitro conditions. In vivo growth of human glioblastoma xenografts was determined in mice. The expression of RFX1 and CD44 in human glioblastoma tissues was quantified.

resultsA putative RFX1 binding sequence existed in the first exon of the human CD44 gene. The transcription activity of the DNA fragment containing this putative sequence was decreased in cells overexpressing RFX1. Regulatory factor X1 bound to the CD44 gene in glioblastoma cells. It reduced CD44 expression and activated Akt and extracellular signal-regulated kinase, signaling molecules downstream of CD44 to regulate cell proliferation and survival. Overexpression of RFX1 inhibited the survival, proliferation, and transwell invasion of glioblastoma cells and in vivo growth of human glioblastoma xenografts. CD44 overexpression reversed RFX1 effects on cell proliferation. Finally, CD44 protein levels were inversely correlated with RFX1 protein levels in human glioblastoma tissues.

conclusionsThese results suggest that RFX1 directly regulates CD44 expression. This mechanism may contribute to RFX1's effects on proliferation, survival, and invasion of glioblastoma cells. Our results provide initial evidence that RFX1 may be an important target/regulator of the malignancy of glioblastoma.

Indexed as

AnimalsCell Line, TumorCell ProliferationDNA-Binding ProteinsDown-RegulationExtracellular Signal-Regulated MAP KinasesGlioblastomaHumansHyaluronan ReceptorsRegulatory Factor X1Regulatory Factor X Transcription FactorsSignal TransductionTranscription FactorsCD44 protein, humanCd44 protein, mouseDNA-Binding ProteinsExtracellular Signal-Regulated MAP KinasesHyaluronan ReceptorsRegulatory Factor X1Regulatory Factor X Transcription FactorsRFX1 protein, humanRfx1 protein, mouseTranscription FactorsCD44glioblastomaregulatory factor X1

Identifiers

PMID24526308
PMCPMC4096175
OpenAlexW2143744820

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.