ArticleRNA biology2014
Increased sensitivity and accuracy of a single-stranded DNA splint-mediated ligation assay (sPAT) reveals poly(A) tail length dynamics of developmentally regulated mRNAs.
Article in RNA biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Poly(A) tail regulation in stem cells and early development.Cell regeneration (London, England) · 2025Review
- Measurement of Poly A Tail Length from Drosophila Larva Brain and Cell Line.Journal of visualized experiments : JoVE · 2024Article
- Measuring the tail: Methods for poly(A) tail profiling.Wiley interdisciplinary reviews. RNA · 2023Review
- Functions and mechanisms of RNA tailing by metazoan terminal nucleotidyltransferases.Wiley interdisciplinary reviews. RNA · 2021Review
- Polyadenylation is the key aspect of GLD-2 function inRNA (New York, N.Y.) · 2017Article
- Essential role for non-canonical poly(A) polymerase GLD4 in cytoplasmic polyadenylation and carbohydrate metabolism.Nucleic acids research · 2017Article
- Transcriptome-wide effects of inverted SINEs on gene expression and their impact on RNA polymerase II activity.Genome biology · 2016Article
- Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Analysis of circadian regulation of poly(A)-tail length.Methods in enzymology · 2015Article
- Kleat: cleavage site analysis of transcriptomes.Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing · 2015Article
- The cytoplasmic poly(A) polymerases GLD-2 and GLD-4 promote general gene expression via distinct mechanisms.Nucleic acids research · 2014Article
- GLD-4-mediated translational activation regulates the size of the proliferative germ cell pool in the adult C. elegans germ line.PLoS genetics · 2014Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly(A) tail length is a readout of an mRNA's translatability and stability, especially in developmental systems. PolyAdenylation Test (PAT) assays attempt to quickly measure the average poly(A) tail length of RNAs of experimental interest. Here we present sPAT, splint-mediated PAT, a procedure that uses a DNA splint to aid in the ligation of an RNA-tag to the poly(A) tail of an mRNA. In comparison to other PAT methodologies, including ePAT, sPAT is highly sensitive to low-abundance mRNAs, gives a more accurate profile of the poly(A) tail distribution, and requires little starting material. To demonstrate its strength, we calibrated sPAT on defined poly(A) tails of synthetic mRNAs, reassessed developmentally regulated poly(A) tail-length changes of known mRNAs from established model organisms, and extended it to the emerging evolutionary developmental nematode model Pristionchus pacificus. Lastly, we used sPAT to analyze the contribution of the two cytoplasmic poly(A) polymerases GLD-2 and GLD-4, and the deadenylase CCR-4, onto Caenorhabditis elegans gld-1 mRNA that encodes a translationally controlled tumor suppressor whose poly(A) tail length measurement proved elusive.
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