ArticleRNA biology2014
Brahma regulates a specific trans-splicing event at the mod(mdg4) locus of Drosophila melanogaster.
Article in RNA biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Multiple intron motifs have redundant functions in the trans-splicing of the mod(mdg4) locus.Nucleic acids research · 2026Article
- Trans-Splicing Enhances Processivity of RNA Polymerase II and Suppresses Polyadenylation Signals.Doklady. Biochemistry and biophysics · 2026Article
- Promoter Specificity and Transcription Levels ModulateInternational journal of molecular sciences · 2025Article
- Article
- The chromatin remodeler ZmCHB101 impacts alternative splicing contexts in response to osmotic stress.Plant cell reports · 2019Article
- Conserved sequences in the Drosophila mod(mdg4) intron promote poly(A)-independent transcription termination and trans-splicing.Nucleic acids research · 2018Article
- SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3' end processing.Nucleic acids research · 2018Article
- SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma.BMC genomics · 2018Article
- Review
- Multiple interactions are involved in a highly specific association of the Mod(mdg4)-67.2 isoform with the Su(Hw) sites inOpen biology · 2017Article
- Coupling of RNA Polymerase II Transcription Elongation with Pre-mRNA Splicing.Journal of molecular biology · 2016Review
- Mod(mdg4)-58.8, isoform of mod(mdg4) loci, directly interacts with MTACP1A and MTACP1B proteins of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2016Article
- A conserved intronic U1 snRNP-binding sequence promotes trans-splicing in Drosophila.Genes & development · 2015Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mod(mdg4) locus of Drosophila melanogaster contains several transcription units encoded on both DNA strands. The mod(mdg4) pre-mRNAs are alternatively spliced, and a very significant fraction of the mature mod(mdg4) mRNAs are formed by trans-splicing. We have studied the transcripts derived from one of the anti-sense regions within the mod(mdg4) locus in order to shed light on the expression of this complex locus. We have characterized the expression of anti-sense mod(mdg4) transcripts in S2 cells, mapped their transcription start sites and cleavage sites, identified and quantified alternatively spliced transcripts, and obtained insight into the regulation of the mod(mdg4) trans-splicing. In a previous study, we had shown that the alternative splicing of some mod(mdg4) transcripts was regulated by Brahma (BRM), the ATPase subunit of the SWI/SNF chromatin-remodeling complex. Here we show, using RNA interference and overexpression of recombinant BRM proteins, that the levels of BRM affect specifically the abundance of a trans-spliced mod(mdg4) mRNA isoform in both S2 cells and larvae. This specific effect on trans-splicing is accompanied by a local increase in the density of RNA polymerase II and by a change in the phosphorylation state of the C-terminal domain of the large subunit of RNA polymerase II. Interestingly, the regulation of the mod(mdg4) splicing by BRM is independent of the ATPase activity of BRM, which suggests that the mechanism by which BRM modulates trans-splicing is independent of its chromatin-remodeling activity.
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