ArticleRNA biology2014
Sustained stabilization of Interleukin-8 mRNA in human macrophages.
Article in RNA biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Interleukin-8 in health and disease.Molecular biomedicine · 2026Review
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- Spaceflight alters molecular networks linked to diverse human diseases in a single cellular model.Science advances · 2026Article
- linc-ADAIN, a human adipose lincRNA, regulates adipogenesis by modulating KLF5 and IL-8 mRNA stability.Cell reports · 2024Article
- Identification, Expression and Antimicrobial Functional Analysis of Interleukin-8 (IL-8) in Response toAnimals : an open access journal from MDPI · 2024Article
- Interlaboratory comparison of an intestinal triple culture to confirm transferability and reproducibility.In vitro models · 2023Article
- The Formulation of the N-Acetylglucosamine as Nanoparticles Increases Its Anti-Inflammatory Activities: An In Vitro Study.Bioengineering (Basel, Switzerland) · 2023Article
- Controlling Macrophage Polarization to Modulate Inflammatory Cues Using Immune-Switch Nanoparticles.International journal of molecular sciences · 2022Article
- Article
- Extracellular Vesicles fromPathogens (Basel, Switzerland) · 2022Article
- Effect of intrabronchial administration of autologous adipose-derived mesenchymal stem cells on severe equine asthma.Stem cell research & therapy · 2022Article
- Human Cytomegalovirus RNA2.7 Is Required for Upregulating Multiple Cellular Genes To Promote Cell Motility and Viral Spread Late in Lytic Infection.Journal of virology · 2021Article
- Nanoparticles for Directed Immunomodulation: Mannose-Functionalized Glycodendrimers Induce Interleukin-8 in Myeloid Cell Lines.Biomacromolecules · 2021Article
- Cytokines: From Clinical Significance to Quantification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Membrane-associated RING-CH (MARCH) 1 and 2 are MARCH family members that inhibit HIV-1 infection.The Journal of biological chemistry · 2019Article
- Bi-phased regulation of the post-transcriptional inflammatory response by Tristetraprolin levels.RNA biology · 2019Article
- The aryl hydrocarbon receptor and interferon gamma generate antiviral states via transcriptional repression.eLife · 2018Article
- Tip110/SART3 regulates IL-8 expression and predicts the clinical outcomes in melanoma.Molecular cancer · 2018Article
- Effects of α-conotoxin ImI on TNF-α, IL-8 and TGF-β expression by human macrophage-like cells derived from THP-1 pre-monocytic leukemic cells.Scientific reports · 2017Article
- Transcriptional profiles of JIA patient blood with subsequent poor response to methotrexate.Rheumatology (Oxford, England) · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mRNAs of most inflammatory mediators are short-lived due to AU-rich elements (AREs) in their 3'-untranslated regions. AREs ensure a low basal level of expression during homeostasis and a transient nature of expression during the inflammatory response. Here, we report that the mRNA of the pro-inflammatory chemokine IL-8, which contains an archetypal ARE, is unexpectedly constitutively abundant and highly stable in primary human monocytes and macrophages. Using the pre-monocyte-like THP-1 cell line that can differentiate into macrophage-like cells, we show that a low level of unstable IL-8 mRNA in undifferentiated cells (half-life<30 min) becomes constitutively elevated and the mRNA is dramatically stabilized in differentiated THP-1 cells with a half-life of more than 15 h similar to primary monocytes and macrophages. In contrast, the level and stability of TNF-α mRNA also containing an ARE is only slightly affected by differentiation; it remains low and unstable in primary macrophages and differentiated THP-1 cells with an estimated half-life of less than 20 min. This differentiation-dependent stabilization of IL-8 mRNA is p38 MAPK-independent and is probably coupled with reduced protein translation. Reporter assays in THP-1 cells suggest that the ARE alone is not sufficient for the constitutive stabilization in macrophage-like cells and imply an effect of the natural biogenesis of the transcript on the stabilization of the mature form. We present a novel, cell type-dependent sustained stabilization of an ARE-containing mRNA with similarities to situations found in disease.
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