ArticleNature communications2014
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.
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Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 300 papers.
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300 citing papers in PubMed, 591 citations in OpenAlex.
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- Review
240 more citing papers are in PubMed but not listed here.
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Authors and funding
37 authors at 9 institutions in 3 countries.
Uwe WeierstallDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Daniel JamesDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Chong WangThe Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
Thomas A WhiteCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
Dingjie WangDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Wei LiuThe Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
John C H SpenceDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
R Bruce DoakDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Garrett NelsonDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Petra FrommeDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
Raimund FrommeDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
Ingo GrotjohannDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
Christopher KupitzDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
Nadia A ZatsepinDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Haiguang LiuDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Shibom BasuDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
Daniel WackerThe Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
Gye Won HanThe Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
Vsevolod KatritchThe Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
Sébastien BoutetSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Marc MesserschmidtSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Garth J WilliamsSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Jason E KoglinSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
M Marvin Seibert1] SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA [2] Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
Markus Klinker1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany [2] Departamento de Química, Universidad Autónoma de Madrid, Ciudad Universitaria de Cantoblanco, Madrid 28049, Spain.
Cornelius GatiCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
Robert L ShoemanMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, Heidelberg 69120, Germany.
Anton BartyCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
Henry N Chapman1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany [2] Department of Physics, University of Hamburg, Hamburg 22761, Germany [3] Center for Ultrafast Imaging, Hamburg 22607, Germany.
Richard A Kirian1] Department of Physics, Arizona State University, Tempe, Arizona 85287, USA [2] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
Kenneth R BeyerleinCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
Raymond C StevensThe Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
Dianfan LiSchool of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.
Syed T A ShahSchool of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.
Nicole HoweSchool of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.
Martin CaffreySchool of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.
Vadim CherezovThe Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
Arizona State University · USScripps Research Institute · USMenlo School · USTrinity College Dublin · IEUniversität Hamburg · DECenter for Free-Electron Laser Science · DEMax Planck Institute for Medical Research · DEMax Planck Institute for the Structure and Dynamics of Matter · DESLAC National Accelerator Laboratory · US
Funding
JCSG Center:Innovative Membrane Protein Technology (RMI)P50GM073197 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI STEVENS, RAYMOND C · 2004 to 2013
$26.8MYeast ExpressionP50GM073210 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STROUD, ROBERT M · 2004 to 2013
$21.5MProject 3U54GM094618 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KUHN, PETER · 2010 to 2014
$17.1MProject 6 (Holton)U54GM094625 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STROUD, ROBERT M · 2010 to 2014
$8.0MCenter for the Rational Design of Membrane Protein CrystallographyU54GM094599 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2014
$6.8MFemtosecond nano-crystallography of membrane proteinsR01GM095583 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2023
$4.3MNIGMS NIH HHS P50 GM073197NIGMS NIH HHS P50 GM073210NIGMS NIH HHS R01 GM095583NIGMS NIH HHS U54 GM094599NIGMS NIH HHS U54 GM094618NIGMS NIH HHS U54 GM094625
Abstract
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
Indexed as
CrystallographyLipidsMembrane ProteinsReceptors, G-Protein-CoupledLipidsMembrane ProteinsReceptors, G-Protein-Coupled
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