ArticleDevelopmental cell2014
Lipid modulation of calcium flux through CaV2.3 regulates acrosome exocytosis and fertilization.
Article in Developmental cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- The systems that transport ions and their impact on sperm function and male fertility.Function (Oxford, England) · 2026Review
- Sperm capacitation ability, as evaluated by Cap-Score™, has little impact on ICSI success.Journal of assisted reproduction and genetics · 2026Article
- Molecular Partners of Voltage-Gated Calcium Channel β and αThe Journal of membrane biology · 2026Review
- Overcoming Batch Variability: A Chemically Defined XRS-Based System for Ovine In Vitro Embryo Production.Veterinary medicine international · 2026Article
- Membrane Fusions During Mammalian Fertilization.Advances in experimental medicine and biology · 2026Review
- A special perspective on human fertilization: The role of sperm capacitation proteins and channels: A narrative review.International journal of reproductive biomedicine · 2025Review
- Bovine serum albumin-induced calcium influx triggers soluble adenylyl cyclase activation and cyclic AMP signalling pathways in mouse sperm capacitation.The Journal of physiology · 2025Article
- Real-Time Imaging of Calcium Dynamics in Human Sperm After Precise Single-Cell Stimulation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm.Frontiers in cell and developmental biology · 2024Review
- Segmental differentiation of the murine epididymis: identification of segment-specific, GM1-enriched vesicles and regulation by luminal fluid factors†.Biology of reproduction · 2023Article
- CatSper Calcium Channels: 20 Years On.Physiology (Bethesda, Md.) · 2023Review
- Testis-enriched ferlin, FER1L5, is required for CaScience advances · 2023Article
- A genetically targeted sensor reveals spatial and temporal dynamics of acrosomal calcium and sperm acrosome exocytosis.The Journal of biological chemistry · 2022Article
- Breeding of CaScientific reports · 2021Article
- High-coverage targeted lipidomics revealed dramatic lipid compositional changes in asthenozoospermic spermatozoa and inverse correlation of ganglioside GM3 with sperm motility.Reproductive biology and endocrinology : RB&E · 2021Article
- Sperm ion channels and transporters in male fertility and infertility.Nature reviews. Urology · 2021Review
- CaChannels (Austin, Tex.) · 2020Review
- HVCN1 Channels Are Relevant for the Maintenance of Sperm Motility During In Vitro Capacitation of Pig Spermatozoa.International journal of molecular sciences · 2020Article
- Release of Porcine Sperm from Oviduct Cells is Stimulated by Progesterone and Requires CatSper.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Membrane lipid regulation of cell function is poorly understood. In early development, sterol efflux and the ganglioside GM1 regulate sperm acrosome exocytosis (AE) and fertilization competence through unknown mechanisms. Here, we show that sterol efflux and focal enrichment of GM1 trigger Ca(2+) influx necessary for AE through CaV2.3, whose activity has been highly controversial in sperm. Sperm lacking CaV2.3's pore-forming α1E subunit showed altered Ca(2+) responses, reduced AE, and a strong subfertility phenotype. Surprisingly, AE depended on spatiotemporal information encoded by flux through CaV2.3, not merely the presence/amplitude of Ca(2+) waves. Using studies in both sperm and voltage clamp of Xenopus oocytes, we define a molecular mechanism for GM1/CaV2.3 regulatory interaction, requiring GM1's lipid and sugar components and CaV2.3's α1E and α2δ subunits. Our results provide a mechanistic understanding of membrane lipid regulation of Ca(2+) flux and therefore Ca(2+)-dependent cellular and developmental processes such as exocytosis and fertilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.