ArticleClinical immunology (Orlando, Fla.)2014
High-throughput sequencing of natively paired antibody chains provides evidence for original antigenic sin shaping the antibody response to influenza vaccination.
Article in Clinical immunology (Orlando, Fla.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
71 citing papers in PubMed, 107 citations in OpenAlex.
- Mechanisms Underlying the Induction of Immunological Imprinting by RNA Viruses and Intervention Strategies.Viruses · 2026Review
- Biological mechanisms of pulmonary inflammation and its association with seropositive rheumatoid arthritis.Frontiers in immunology · 2025Review
- A candidate antibody drug for prevention of malaria.Nature medicine · 2024Article
- Affinity-matured homotypic interactions induce spectrum of PfCSP structures that influence protection from malaria infection.Nature communications · 2023Article
- Immune Imprinting and Implications for COVID-19.Vaccines · 2023Review
- Understanding repertoire sequencing data through a multiscale computational model of the germinal center.NPJ systems biology and applications · 2023Article
- Bioinformatic Analysis of Natively Paired VH:VL Antibody Repertoires for Antibody Discovery.Methods in molecular biology (Clifton, N.J.) · 2023Article
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- A novel CSP C-terminal epitope targeted by an antibody with protective activity against Plasmodium falciparum.PLoS pathogens · 2022Article
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- The deficiency in Th2-like Tfh cells affects the maturation and quality of HIV-specific B cell response in viremic infection.Frontiers in immunology · 2022Article
- Mining HIV controllers for broad and functional antibodies to recognize and eliminate HIV-infected cells.Cell reports · 2021Article
- Original Antigenic Sin: How Original? How Sinful?Cold Spring Harbor perspectives in medicine · 2021Review
- Distinct clonal evolution of B-cells in HIV controllers with neutralizing antibody breadth.eLife · 2021Article
- Convergent antibody evolution and clonotype expansion following influenza virus vaccination.PloS one · 2021Article
- Modeling human adaptive immune responses with tonsil organoids.Nature medicine · 2021Article
- Using B cell receptor lineage structures to predict affinity.PLoS computational biology · 2020Article
- Retrospective Assessment of the Antigenic Similarity of Egg-Propagated and Cell Culture-Propagated Reference Influenza Viruses as Compared with Circulating Viruses across Influenza Seasons 2002-2003 to 2017-2018.International journal of environmental research and public health · 2020Article
- A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease.The Journal of infectious diseases · 2020Article
- Structure and mechanism of monoclonal antibody binding to the junctional epitope of Plasmodium falciparum circumsporozoite protein.PLoS pathogens · 2020Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We developed a DNA barcoding method to enable high-throughput sequencing of the cognate heavy- and light-chain pairs of the antibodies expressed by individual B cells. We used this approach to elucidate the plasmablast antibody response to influenza vaccination. We show that >75% of the rationally selected plasmablast antibodies bind and neutralize influenza, and that antibodies from clonal families, defined by sharing both heavy-chain VJ and light-chain VJ sequence usage, do so most effectively. Vaccine-induced heavy-chain VJ regions contained on average >20 nucleotide mutations as compared to their predicted germline gene sequences, and some vaccine-induced antibodies exhibited higher binding affinities for hemagglutinins derived from prior years' seasonal influenza as compared to their affinities for the immunization strains. Our results show that influenza vaccination induces the recall of memory B cells that express antibodies that previously underwent affinity maturation against prior years' seasonal influenza, suggesting that 'original antigenic sin' shapes the antibody response to influenza vaccination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.