ArticleJournal of pathology informatics2013
Optimal z-axis scanning parameters for gynecologic cytology specimens.
Article in Journal of pathology informatics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Relevance of the College of American Pathologists guideline for validating whole slide imaging for diagnostic purposes to cytopathology.Cytopathology : official journal of the British Society for Clinical Cytology · 2023Pooled it
- Quantitative Assessment of Focus Quality in Whole-Slide Imaging of Thyroid Liquid-Based Cytology Using Laplacian Variance.Endocrine pathology · 2025Article
- Utility of whole-slide imaging for rapid evaluation of thyroid FNA: A multireader prospective study.Cancer cytopathology · 2025Article
- Automated recognition and segmentation of lung cancer cytological images based on deep learning.PloS one · 2025Article
- Rapid 3D imaging at cellular resolution for digital cytopathology with a multi-camera array scanner (MCAS).Npj imaging · 2024Article
- A deep learning model to triage and predict adenocarcinoma on pancreas cytology whole slide imaging.Scientific reports · 2023Article
- Recent Application of Artificial Intelligence in Non-Gynecological Cancer Cytopathology: A Systematic Review.Cancers · 2022Review
- Comparative Assessment of Digital Pathology Systems for Primary Diagnosis.Journal of pathology informatics · 2021Article
- Experience Reviewing Digital Pap Tests using a Gallery of Images.Journal of pathology informatics · 2021Article
- Eye tracking reveals expertise-related differences in the time-course of medical image inspection and diagnosis.Journal of medical imaging (Bellingham, Wash.) · 2020Article
- From Scope to Screen: The Evolution of Histology Education.Advances in experimental medicine and biology · 2020Article
- Video compression to support the expansion of whole-slide imaging into cytology.Journal of medical imaging (Bellingham, Wash.) · 2019Article
- Constant Quest for Quality: Digital Cytopathology.Journal of pathology informatics · 2018Article
- Super-Resolution Digital Pathology Image Processing of Bone Marrow Aspirate and Cytology Smears and Tissue Sections.Journal of pathology informatics · 2018Article
- Utilization of virtual microscopy in cytotechnology educational programs in the United States.Journal of pathology informatics · 2016Article
- Removing defocused objects from single focal plane scans of cytological slides.Journal of pathology informatics · 2016Article
- Investigation of scanning parameters for thyroid fine needle aspiration cytology specimens: A pilot study.Journal of pathology informatics · 2015Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe use of virtual microscopy (VM) in clinical cytology has been limited due to the inability to focus through three dimensional (3D) cell clusters with a single focal plane (2D images). Limited information exists regarding the optimal scanning parameters for 3D scanning.
aimsThe purpose of this study was to determine the optimal number of the focal plane levels and the optimal scanning interval to digitize gynecological (GYN) specimens prepared on SurePath™ glass slides while maintaining a manageable file size. SUBJECTS AND
methodsThe iScanCoreo Au scanner (Ventana, AZ, USA) was used to digitize 192 SurePath™ glass slides at three focal plane levels at 1 μ interval. The digitized virtual images (VI) were annotated using BioImagene's Image Viewer. Five participants interpreted the VI and recorded the focal plane level at which they felt confident and later interpreted the corresponding glass slide specimens using light microscopy (LM). The participants completed a survey about their experiences. Inter-rater agreement and concordance between the VI and the glass slide specimens were evaluated.
resultsThis study determined an overall high intra-rater diagnostic concordance between glass and VI (89-97%), however, the inter-rater agreement for all cases was higher for LM (94%) compared with VM (82%). Survey results indicate participants found low grade dysplasia and koilocytes easy to diagnose using three focal plane levels, the image enhancement tool was useful and focusing through the cells helped with interpretation; however, the participants found VI with hyperchromatic crowded groups challenging to interpret. Participants reported they prefer using LM over VM. This study supports using three focal plane levels and 1 μ interval to expand the use of VM in GYN cytology.
conclusionFuture improvements in technology and appropriate training should make this format a more preferable and practical option in clinical cytology.
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