ArticleJournal of parasitology research2014
A comparison of nested PCR assay with conventional techniques for diagnosis of intestinal cryptosporidiosis in AIDS cases from northern India.
Article in Journal of parasitology research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 43 citations in OpenAlex.
- Cryptosporidiosis in HIV-positive patients and related risk factors: A systematic review and meta-analysis.Parasite (Paris, France) · 2020Pooled it
- Pooled it
- Examination of ELISA against PCR for assessing treatment efficacy against Cryptosporidium in a clinical trial context.PloS one · 2023Trial
- Therapeutic effects of acetylspiramycin and garlicin on cryptosporidiosis among drug users.International journal for parasitology. Drugs and drug resistance · 2015Trial
- Detection and genetic characterization of the GP60 Gene of Cryptosporidium parvum isolated from HIVGut pathogens · 2026Article
- Development of Multiplex Real-Time Quantitative PCR for the Detection ofAnimals : an open access journal from MDPI · 2026Article
- Clinical cases ofJournal of veterinary research · 2026Article
- Diagnostic methods for protozoan diseases: a review focused on leishmaniasis, Chagas disease and malaria.Frontiers in microbiology · 2026Review
- Role of Cryptosporidium spp in Development of Colorectal Cancer.Asian Pacific journal of cancer prevention : APJCP · 2023Article
- Article
- Diagnosis and control of cryptosporidiosis in farm animals.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Article
- Development and Preliminary Application of a Triplex Real-Time Quantitative PCR Assay for the Simultaneous Detection ofFrontiers in microbiology · 2022Article
- Molecular prevalence ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Review
- Detection ofThe Korean journal of parasitology · 2018Article
- Diagnostic biomarkers in murineJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Prevalence and Genetic Characterization of Cryptosporidium Species in Dairy Calves in Central Ethiopia.PloS one · 2016Article
- Cryptosporidium prevalence and risk factors among mothers and infants 0 to 6 months in rural and semi-rural Northwest Tanzania: a prospective cohort study.PLoS neglected tropical diseases · 2014Article
- The Impact of Different Copro-preservation Conditions on Molecular Detection ofIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptosporidiosis is a very important opportunistic infection and is responsible for significant morbidity and mortality in HIV/AIDS patients. Although current laboratory methods are generally considered adequate to detect high concentrations of oocysts, they fail to detect cases of cryptosporidiosis in many immunocompromised patients. The present study was done to determine the diagnostic efficacy of modified Ziehl-Neelsen (ZN), antigen detection ELISA, and a nested PCR assay for detection of Cryptosporidium in 58 adult AIDS cases with diarrhea from the ART clinic of Lok Nayak Hospital, New Delhi. Cryptosporidium was detected in 17 (29.4%), 39 (67.3%), and 45 (77.5%) cases by modified ZN staining, antigen ELISA, and nested PCR assay, respectively. Taking nested PCR as the gold standard, specificity of both modified ZN staining and Cryptosporidium antigen detection ELISA was 100% while the sensitivity of the tests was 37.8% and 86.6%, respectively. PCR was more sensitive than the other two diagnostic modalities but required a more hands-on time per sample and was more expensive than microscopy. PCR, however, was very adaptable to batch analysis, reducing the costs considerably. This assay can therefore have considerable advantages in the treatment of immunosuppressed individuals like AIDS patients, allowing their early diagnosis and decreasing the morbidity and the mortality.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.