Evidence map›Paper›PMID 24523899›Full record

ArticlePloS one2014

De novo assembly and transcriptome analysis of contrasting sugarcane varieties.

Claudio Benicio Cardoso-Silva, Estela Araujo Costa, Melina Cristina Mancini, Thiago Willian Almeida Balsalobre, Lucas Eduardo Costa Canesin, Luciana Rossini Pinto, Monalisa Sampaio Carneiro, Antonio Augusto Franco Garcia, Anete Pereira de Souza, Renato Vicentini

Abstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed.

  1. Adaptive Responses of Tropical Crops: A Multi-Scale Omics Integrated Perspective.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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  15. Frontiers in plant science · 2021
    Article
  16. Article
  17. Leaping into the Unknown World ofJournal of fungi (Basel, Switzerland) · 2020
    Article
  18. Article
  19. Article
  20. Review

8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Claudio Benicio Cardoso-SilvaCenter for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
Estela Araujo CostaCenter for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
Melina Cristina ManciniCenter for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
Thiago Willian Almeida BalsalobreCenter for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
Lucas Eduardo Costa CanesinCenter for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.
Luciana Rossini PintoCentro Avançado da Pesquisa Tecnológica do Agronegócio de Cana (IAC/Apta), Ribeirão Preto, SP, Brazil.
Monalisa Sampaio CarneiroDepartamento de Biotecnologia e Produção Vegetal e Animal, Centro de Ciências Agrárias, Universidade Federal de São Carlos, Araras, SP, Brazil.
Antonio Augusto Franco GarciaDepartamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil.
Anete Pereira de SouzaCenter for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil ; Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
Renato VicentiniCenter for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sugarcane is an important crop and a major source of sugar and alcohol. In this study, we performed de novo assembly and transcriptome annotation for six sugarcane genotypes involved in bi-parental crosses. The de novo assembly of the sugarcane transcriptome was performed using short reads generated using the Illumina RNA-Seq platform. We produced more than 400 million reads, which were assembled into 72,269 unigenes. Based on a similarity search, the unigenes showed significant similarity to more than 28,788 sorghum proteins, including a set of 5,272 unigenes that are not present in the public sugarcane EST databases; many of these unigenes are likely putative undescribed sugarcane genes. From this collection of unigenes, a large number of molecular markers were identified, including 5,106 simple sequence repeats (SSRs) and 708,125 single-nucleotide polymorphisms (SNPs). This new dataset will be a useful resource for future genetic and genomic studies in this species.

Indexed as

TranscriptomeCluster AnalysisDatabases, GeneticExpressed Sequence TagsGenes, PlantGenetic MarkersGenotypeMicrosatellite RepeatsOpen Reading FramesPolymorphism, Single NucleotideSaccharumSequence Analysis, DNASucroseGenetic MarkersSucrose

Identifiers

PMID24523899
PMCPMC3921171

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.