ArticlePloS one2014
Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Salt-sensitive hypertension in GR mutant rats is associated with altered plasma polyunsaturated fatty acid levels and aortic vascular reactivity.Pflugers Archiv : European journal of physiology · 2025Article
- Role of glucocorticoid receptor mutations in hypertension and adrenal gland hyperplasia.Pflugers Archiv : European journal of physiology · 2022Review
- Recent Advances in the Production of Genome-Edited Rats.International journal of molecular sciences · 2022Review
- Salt-Sensitive Hypertension in GRInternational journal of molecular sciences · 2021Article
- CRISPR/Cas9-mediated correction ofMolecular therapy. Nucleic acids · 2021Article
- Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models.Frontiers in genetics · 2021Review
- A Universal Surrogate Reporter for Efficient Enrichment of CRISPR/Cas9-Mediated Homology-Directed Repair in Mammalian Cells.Molecular therapy. Nucleic acids · 2020Article
- Animal Models of Hypertension: A Scientific Statement From the American Heart Association.Hypertension (Dallas, Tex. : 1979) · 2019Review
- An efficient platform for generating somatic point mutations with germline transmission in the zebrafish by CRISPR/Cas9-mediated gene editing.The Journal of biological chemistry · 2018Article
- The Role of Gene Editing in Neurodegenerative Diseases.Cell transplantation · 2018Review
- From engineering to editing the rat genome.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Gene editing in birds takes flight.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- From integrative genomics to systems genetics in the rat to link genotypes to phenotypes.Disease models & mechanisms · 2016Review
- ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.Nature communications · 2016Article
- Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins.Scientific reports · 2015Article
- Article
- The utility of Apc-mutant rats in modeling human colon cancer.Disease models & mechanisms · 2014Review
- Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases.Genome research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.