ArticlePloS one2014
Dormant cells of Staphylococcus aureus are resuscitated by spent culture supernatant.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Genomic insights into specialized and primary metabolism gene clusters in methicillin-resistant Staphylococcus aureus from Egyptian community settings.BMC genomics · 2026Article
- Methicillin-sensitiveMicrobial genomics · 2025Article
- Bacterial persisters: molecular mechanisms and therapeutic development.Signal transduction and targeted therapy · 2024Review
- Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights.Frontiers in cellular and infection microbiology · 2023Article
- Roles of viable but non-culturable state in the survival ofFrontiers in cellular and infection microbiology · 2023Article
- The crafty opponent: the defense systems of Staphylococcus aureus and response measures.Folia microbiologica · 2022Review
- Generation of Distinct Differentially Culturable Forms ofMicrobiology spectrum · 2022Article
- Formic acid, an organic acid food preservative, induces viable-but-non-culturable state, and triggers new Antimicrobial Resistance traits inFrontiers in microbiology · 2022Article
- The Impact of Protease during Recovery from Viable but Non-Culturable (VBNC) State inMicroorganisms · 2021Article
- Dynamic PET-facilitated modeling and high-dose rifampin regimens forScience translational medicine · 2021Article
- Insights into the global effect on Staphylococcus aureus growth arrest by induction of the endoribonuclease MazF toxin.Nucleic acids research · 2020Article
- Persisters of Klebsiella pneumoniae and Proteus mirabilis: A Common Phenomenon and Different Behavior Profiles.Current microbiology · 2020Article
- Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli.Scientific reports · 2019Article
- Quorum Sensing-Regulated Phenol-Soluble Modulins Limit Persister Cell Populations inFrontiers in microbiology · 2018Article
- Glucose Augments Killing Efficiency of Daptomycin Challenged Staphylococcus aureus Persisters.PloS one · 2016Article
- Control of Biofilms with the Fatty Acid Signaling Molecule cis-2-Decenoic Acid.Pharmaceuticals (Basel, Switzerland) · 2015Review
- Persistent Staphylococcus aureus isolates from two independent cases of bacteremia display increased bacterial fitness and novel immune evasion phenotypes.Infection and immunity · 2015Article
- Structure of the stationary phase survival protein YuiC from B.subtilis.BMC structural biology · 2015Article
- The fatty acid signaling molecule cis-2-decenoic acid increases metabolic activity and reverts persister cells to an antimicrobial-susceptible state.Applied and environmental microbiology · 2014Article
- Role of daptomycin in the induction and persistence of the viable but non-culturable state of Staphylococcus aureus biofilms.Pathogens (Basel, Switzerland) · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can be resuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturable counts in dormant cultures of S. aureus SH1000 fell from 10(6)-10(7) cfu/ml to <10 cfu/ml as measured by the Most Probable Number method in liquid culture, while total counts as determined by microscopy, and supported by data from RT-qPCR, remained around 10(6)-10(7) cells/ml. Supplementing cultures with 25-50% spent medium resulted in a >600-fold increase in bacterial growth. Resuscitation was a specific effect, greatly reduced by boiling or addition of trypsin to the spent supernatant. Supernatant also effected a reduction in lag phase of dormant cultures. SEM demonstrated the presence of small coccoid cells in dormant cultures. The results are similar to those seen with resuscitation promoting factors (Rpfs) in actinobacteria. This is the first time resuscitation has been demonstrated in Staphylococcus aureus, which is an important human pathogen. A better understanding of control and reactivation of dormant cells could lead to major improvements in managing staphylococcal infections; resuscitation could be an important step in restoring susceptibility to antibiotic treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.