ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
The transcription factors islet and Lim3 combinatorially regulate ion channel gene expression.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
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- Synergistic activation by Glass and Pointed promotes neuronal identity in the Drosophila eye disc.Nature communications · 2024Article
- Neuronal identity control at the resolution of a single transcription factor isoform.bioRxiv : the preprint server for biology · 2024Article
- 40 years of homeodomain transcription factors in the Drosophila nervous system.Development (Cambridge, England) · 2024Review
- Review
- Investigation of Islet2a function in zebrafish embryos: Mutants and morphants differ in morphologic phenotypes and gene expression.PloS one · 2018Article
- Neuronal cell fate specification by the molecular convergence of different spatio-temporal cues on a common initiator terminal selector gene.PLoS genetics · 2017Article
- Islet Coordinately Regulates Motor Axon Guidance and Dendrite Targeting through the Frazzled/DCC Receptor.Cell reports · 2017Article
- In vivo matching of postsynaptic excitability with spontaneous synaptic inputs during formation of the rat calyx of Held synapse.The Journal of physiology · 2017Article
- ISL1-based LIM complexes control Slit2 transcription in developing cranial motor neurons.Scientific reports · 2016Article
- Molecular variability elicits a tunable switch with discrete neuromodulatory response phenotypes.Journal of computational neuroscience · 2016Article
- Transcription factors and effectors that regulate neuronal morphology.Development (Cambridge, England) · 2014Review
- Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability.Neural development · 2014Article
- Beadex function in the motor neurons is essential for female reproduction in Drosophila melanogaster.PloS one · 2014Article
- Transcriptional selectors, masters, and combinatorial codes: regulatory principles of neural subtype specification.Wiley interdisciplinary reviews. Developmental biologyReview
- Dam it's good! DamID profiling of protein-DNA interactions.Wiley interdisciplinary reviews. Developmental biologyReview
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Expression of appropriate ion channels is essential to allow developing neurons to form functional networks. Our previous studies have identified LIM-homeodomain (HD) transcription factors (TFs), expressed by developing neurons, that are specifically able to regulate ion channel gene expression. In this study, we use the technique of DNA adenine methyltransferase identification (DamID) to identify putative gene targets of four such TFs that are differentially expressed in Drosophila motoneurons. Analysis of targets for Islet (Isl), Lim3, Hb9, and Even-skipped (Eve) identifies both ion channel genes and genes predicted to regulate aspects of dendritic and axonal morphology. Significantly, some ion channel genes are bound by more than one TF, consistent with the possibility of combinatorial regulation. One such gene is Shaker (Sh), which encodes a voltage-dependent fast K(+) channel (Kv1.1). DamID reveals that Sh is bound by both Isl and Lim3. We used body wall muscle as a test tissue because in conditions of low Ca(2+), the fast K(+) current is carried solely by Sh channels (unlike neurons in which a second fast K(+) current, Shal, also contributes). Ectopic expression of isl, but not Lim3, is sufficient to reduce both Sh transcript and Sh current level. By contrast, coexpression of both TFs is additive, resulting in a significantly greater reduction in both Sh transcript and current compared with isl expression alone. These observations provide evidence for combinatorial activity of Isl and Lim3 in regulating ion channel gene expression.
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