ArticleJournal of clinical microbiology2014
Identifying Neisseria species by use of the 50S ribosomal protein L6 (rplF) gene.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 63 citations in OpenAlex.
- Clinical Metagenomic Sequencing for Diagnosis of Meningitis and Encephalitis.The New England journal of medicine · 2019Trial
- Pharyngeal carriage of Neisseria species in the African meningitis belt.The Journal of infection · 2016Trial
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- Emergence and evolution of mosaicFrontiers in microbiology · 2024Article
- Impact of HIV on the Oral Microbiome of Children Living in Sub-Saharan Africa, Determined by Using anMicrobiology spectrum · 2023Article
- Whole Genome Analyses Accurately IdentifyInternational journal of molecular sciences · 2022Article
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- Invasive Meningococcal X Disease during the COVID-19 Pandemic, Brazil.Emerging infectious diseases · 2022Article
- WGS of CommensalPathogens (Basel, Switzerland) · 2021Article
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- Persistence Dynamics of Antimicrobial-ResistantAntimicrobial agents and chemotherapy · 2020Article
- Fine-Scale Haplotype Structure Reveals Strong Signatures of Positive Selection in a Recombining Bacterial Pathogen.Molecular biology and evolution · 2020Article
- Neisseria meningitidis: using genomics to understand diversity, evolution and pathogenesis.Nature reviews. Microbiology · 2020Review
- Meningococcal Quinolone Resistance Originated from Several CommensalAntimicrobial agents and chemotherapy · 2020Article
- Oropharyngeal microbiome of a college population following a meningococcal disease outbreak.Scientific reports · 2020Article
- Prevention and Control of Pathogens Based on Big-Data Mining and Visualization Analysis.Frontiers in molecular biosciences · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The comparison of 16S rRNA gene sequences is widely used to differentiate bacteria; however, this gene can lack resolution among closely related but distinct members of the same genus. This is a problem in clinical situations in those genera, such as Neisseria, where some species are associated with disease while others are not. Here, we identified and validated an alternative genetic target common to all Neisseria species which can be readily sequenced to provide an assay that rapidly and accurately discriminates among members of the genus. Ribosomal multilocus sequence typing (rMLST) using ribosomal protein genes has been shown to unambiguously identify these bacteria. The PubMLST Neisseria database (http://pubmlst.org/neisseria/) was queried to extract the 53 ribosomal protein gene sequences from 44 genomes from diverse species. Phylogenies reconstructed from these genes were examined, and a single 413-bp fragment of the 50S ribosomal protein L6 (rplF) gene was identified which produced a phylogeny that was congruent with the phylogeny reconstructed from concatenated ribosomal protein genes. Primers that enabled the amplification and direct sequencing of the rplF gene fragment were designed to validate the assay in vitro and in silico. Allele sequences were defined for the gene fragment, associated with particular species names, and stored on the PubMLST Neisseria database, providing a curated electronic resource. This approach provides an alternative to 16S rRNA gene sequencing, which can be readily replicated for other organisms for which more resolution is required, and it has potential applications in high-resolution metagenomic studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.