ArticleMolecular biology of the cell2014
The Neurospora crassa exocyst complex tethers Spitzenkörper vesicles to the apical plasma membrane during polarized growth.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 92 citations in OpenAlex.
- Article
- Organelle scaling over a 100-fold cell size range.bioRxiv : the preprint server for biology · 2026Article
- Revisiting fungal polarized growth: from Spitzenkörper to crescent accumulation of secretory vesicles.iScience · 2025Article
- Growth conditions shape the proteome and diversity ofCell surface (Amsterdam, Netherlands) · 2025Article
- The C-terminal domain of SEC-10 is fundamental for exocyst function, apical organization, and cell morphogenesis inMolecular biology of the cell · 2025Article
- Regulation of yeast polarized exocytosis by phosphoinositide lipids.Cellular and molecular life sciences : CMLS · 2024Review
- Emerging Roles of Exocyst Complex in Fungi: A Review.Journal of fungi (Basel, Switzerland) · 2024Review
- A palisade-shaped membrane reservoir is required for rapid ring cell inflation in Drechslerella dactyloides.Nature communications · 2023Article
- Looking outside the box: a comparative cross-kingdom view on the cell biology of the three major lineages of eukaryotic multicellular life.Cellular and molecular life sciences : CMLS · 2023Review
- Article
- The type V myosin-containing complex HUM is a RAB11 effector powering movement of secretory vesicles.iScience · 2022Article
- Article
- Article
- The Exocyst Regulates Hydrolytic Enzyme Secretion at Hyphal Tips and Septa in the Banana Fusarium Wilt Fungus Fusarium odoratissimum.Applied and environmental microbiology · 2021Article
- Turning Inside Out: Filamentous Fungal Secretion and Its Applications in Biotechnology, Agriculture, and the Clinic.Journal of fungi (Basel, Switzerland) · 2021Review
- Article
- An exocyst component, Sec5, is essential for ascospore formation inMycoscience · 2021Article
- Omics Approaches for Understanding Biogenesis, Composition and Functions of Fungal Extracellular Vesicles.Frontiers in genetics · 2021Review
- Hex1, the Major Component of Woronin Bodies, Is Required for Normal Development, Pathogenicity, and Stress Response in the Plant Pathogenic FungusJournal of fungi (Basel, Switzerland) · 2020Article
- Spitzenkörper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds.Nature communications · 2020Article
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
Fungal hyphae are among the most highly polarized cells. Hyphal polarized growth is supported by tip-directed transport of secretory vesicles, which accumulate temporarily in a stratified manner in an apical vesicle cluster, the Spitzenkörper. The exocyst complex is required for tethering of secretory vesicles to the apical plasma membrane. We determined that the presence of an octameric exocyst complex is required for the formation of a functional Spitzenkörper and maintenance of regular hyphal growth in Neurospora crassa. Two distinct localization patterns of exocyst subunits at the hyphal tip suggest the dynamic formation of two assemblies. The EXO-70/EXO-84 subunits are found at the peripheral part of the Spitzenkörper, which partially coincides with the outer macrovesicular layer, whereas exocyst components SEC-5, -6, -8, and -15 form a delimited crescent at the apical plasma membrane. Localization of SEC-6 and EXO-70 to the plasma membrane and the Spitzenkörper, respectively, depends on actin and microtubule cytoskeletons. The apical region of exocyst-mediated vesicle fusion, elucidated by the plasma membrane-associated exocyst subunits, indicates the presence of an exocytotic gradient with a tip-high maximum that dissipates gradually toward the subapex, confirming the earlier predictions of the vesicle supply center model for hyphal morphogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.