ArticleOpen biology2014
A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle.
Article in Open biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Structure and function of the centromere and inner kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Transcriptomic analysis reveals lung cancer and subtype-specific alternative splicing biomarkers regulated by RNA-binding proteins.Molecular therapy. Nucleic acids · 2025Article
- CENcyclopedia: dynamic landscape of kinetochore architecture throughout the cell cycle.Nature communications · 2025Article
- Cohesin-mediated stabilization of the CCAN complex at kinetochores in mitosis.Current biology : CB · 2025Article
- Native and tagged CENP-A histones are functionally inequivalent.Epigenetics & chromatin · 2024Article
- Fluorescence complementation-based FRET imaging reveals centromere assembly dynamics.Molecular biology of the cell · 2024Article
- Mobility of kinetochore proteins measured by FRAP analysis in living cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2022Article
- Holliday junction recognition protein interacts with and specifies the centromeric assembly of CENP-T.The Journal of biological chemistry · 2019Article
- High-resolution mapping of centromeric protein association using APEX-chromatin fibers.Epigenetics & chromatin · 2018Article
- Article
- Stepwise unfolding supports a subunit model for vertebrate kinetochores.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Chromatin dynamics during the cell cycle at centromeres.Nature reviews. Genetics · 2017Review
- CENPT bridges adjacent CENPA nucleosomes on young human α-satellite dimers.Genome research · 2016Article
- Kinetochore assembly and function through the cell cycle.Chromosoma · 2016Review
- Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability.Open biology · 2016Article
- The CENP-T C-terminus is exclusively proximal to H3.1 and not to H3.2 or H3.3.International journal of molecular sciences · 2015Article
- The distinct functions of CENP-C and CENP-T/W in centromere propagation and function in Xenopus egg extracts.Nucleus (Austin, Tex.) · 2015Article
- The centromere: epigenetic control of chromosome segregation during mitosis.Cold Spring Harbor perspectives in biology · 2014Review
- Review
- Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functional identity of centromeres arises from a set of specific nucleoprotein particle subunits of the centromeric chromatin fibre. These include CENP-A and histone H3 nucleosomes and a novel nucleosome-like complex of CENPs -T, -W, -S and -X. Fluorescence cross-correlation spectroscopy and Förster resonance energy transfer (FRET) revealed that human CENP-S and -X exist principally in complex in soluble form and retain proximity when assembled at centromeres. Conditional labelling experiments show that they both assemble de novo during S phase and G2, increasing approximately three- to fourfold in abundance at centromeres. Fluorescence recovery after photobleaching (FRAP) measurements documented steady-state exchange between soluble and assembled pools, with CENP-X exchanging approximately 10 times faster than CENP-S (t1/2 ∼ 10 min versus 120 min). CENP-S binding to sites of DNA damage was quite distinct, with a FRAP half-time of approximately 160 s. Fluorescent two-hybrid analysis identified CENP-T as a uniquely strong CENP-S binding protein and this association was confirmed by FRET, revealing a centromere-bound complex containing CENP-S, CENP-X and CENP-T in proximity to histone H3 but not CENP-A. We propose that deposition of the CENP-T/W/S/X particle reveals a kinetochore-specific chromatin assembly pathway that functions to switch centromeric chromatin to a mitosis-competent state after DNA replication. Centromeres shuttle between CENP-A-rich, replication-competent and H3-CENP-T/W/S/X-rich mitosis-competent compositions in the cell cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.