ArticleMolecular plant pathology2014
Sequence diversity in the large subunit of RNA polymerase I contributes to Mefenoxam insensitivity in Phytophthora infestans.
Article in Molecular plant pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 85 citations in OpenAlex.
- High Genotypic Polymorphism and Extensive Gene Flow Drive the Population Dynamics of Potato Oomycete Pathogen,Journal of fungi (Basel, Switzerland) · 2026Article
- Genome Re-Sequencing and Functional Analysis Reveal an α-1,3-Glucosyltransferase Conferring Metalaxyl Resistance inJournal of fungi (Basel, Switzerland) · 2026Article
- Deciphering fungicide resistance in Phytophthora: mechanisms, prevalence, and sustainable management approaches.World journal of microbiology & biotechnology · 2024Review
- Study on the Design, Synthesis, Bioactivity and Translocation of the Conjugates of Phenazine-1-carboxylic Acid andMolecules (Basel, Switzerland) · 2024Article
- Identification of sequence mutations in Phytophthora cactorum genome associated with mefenoxam resistance and development of a molecular assay for the mutant detection in strawberry (F. × ananassa).Scientific reports · 2023Article
- Evaluation of the Ability of Seven Active Ingredients of Fungicides to SuppressJournal of fungi (Basel, Switzerland) · 2022Article
- Fantastic Downy Mildew Pathogens and How to Find Them: Advances in Detection and Diagnostics.Plants (Basel, Switzerland) · 2021Review
- Lauric Acid Is a Potent Biological Control Agent That Damages the Cell Membrane ofFrontiers in microbiology · 2021Article
- 4-Ethylphenol, A Volatile Organic Compound Produced by Disease-Resistant Soybean, Is a Potential Botanical Agrochemical Against Oomycetes.Frontiers in plant science · 2021Article
- Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato.Molecular plant pathology · 2020Article
- Differential Potential ofMicroorganisms · 2020Article
- Sensitivity of Different Developmental Stages and Resistance Risk Assessment ofFrontiers in microbiology · 2020Article
- Biocontrol and Action Mechanism ofInternational journal of molecular sciences · 2019Article
- Evidence of genetically diverse virulent mating types of Phytophthora capsici from Capsicum annum L.World journal of microbiology & biotechnology · 2018Article
- Resistance Assessment for Oxathiapiprolin in Phytophthora capsici and the Detection of a Point Mutation (G769W) in PcORP1 that Confers Resistance.Frontiers in microbiology · 2016Article
- The Top 10 oomycete pathogens in molecular plant pathology.Molecular plant pathology · 2015Review
- Development of a bipartite ecdysone-responsive gene switch for the oomycete Phytophthora infestans and its use to manipulate transcription during axenic culture and plant infection.Molecular plant pathology · 2015Article
- Article
- Variation in capsidiol sensitivity between Phytophthora infestans and Phytophthora capsici is consistent with their host range.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Phenylamide fungicides have been widely used for the control of oomycete-incited plant diseases for over 30 years. Insensitivity to this chemical class of fungicide was recorded early in its usage history, but the precise protein(s) conditioning insensitivity has proven difficult to determine. To determine the genetic basis of insensitivity and to inform strategies for the cloning of the gene(s) responsible, genetic crosses were established between Mefenoxam sensitive and intermediate insensitive isolates of Phytophthora infestans, the potato late blight pathogen. F1 progeny showed the expected semi-dominant phenotypes for Mefenoxam insensitivity and suggested the involvement of multiple loci, complicating the positional cloning of the gene(s) conditioning insensitivity to Mefenoxam. Instead, a candidate gene strategy was used, based on previous observations that the primary effect of phenylamide compounds is to inhibit ribosomal RNA synthesis. The subunits of RNA polymerase I (RNApolI) were sequenced from sensitive and insensitive isolates and F1 progeny. Single nucleotide polymorphisms (SNPs) specific to insensitive field isolates were identified in the gene encoding the large subunit of RNApolI. In a survey of field isolates, SNP T1145A (Y382F) showed an 86% association with Mefenoxam insensitivity. Isolates not showing this association belonged predominantly to one P. infestans genotype. The transfer of the 'insensitive' allele of RPA190 to a sensitive isolate yielded transgenic lines that were insensitive to Mefenoxam. These results demonstrate that sequence variation in RPA190 contributes to insensitivity to Mefenoxam in P. infestans.
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