ArticleJournal of the American Chemical Society2014
New reactions and products resulting from alternative interactions between the P450 enzyme and redox partners.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- P450 engineering via structure-guided rational design achieves high C21-selectivity and bioconversion in steroid biosynthesis.Synthetic and systems biotechnology · 2026Article
- Molecular Basis for Peptide Nitration by a Novel Cytochrome P450 Enzyme in RiPP Biosynthesis.ACS catalysis · 2025Article
- Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis.Engineering microbiology · 2025Article
- Functional analysis of the whole CYPome and Fdxome ofEngineering microbiology · 2024Article
- Structure-Function Analysis of the Biotechnologically Important Cytochrome P450 107 (CYP107) Enzyme Family.Biomolecules · 2023Article
- Prokaryotic expression and characterization of artificial self-sufficient CYP120A monooxygenases.Applied microbiology and biotechnology · 2023Article
- Redox partner recognition and selectivity of cytochrome P450lin (CYP111A1).Journal of inorganic biochemistry · 2023Article
- Differential RNA-Seq Analysis Predicts Genes Related to Terpene Tailoring inPlants (Basel, Switzerland) · 2023Article
- Improved 2α-Hydroxylation Efficiency of Steroids by CYP154C2 Using Structure-Guided Rational Design.Applied and environmental microbiology · 2023Article
- Exploring optimal Taxol® CYP725A4 activity in Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- Current state and future perspectives of cytochrome P450 enzymes for C-H and C=C oxygenation.Synthetic and systems biotechnology · 2022Article
- Three pairs of surrogate redox partners comparison for Class I cytochrome P450 enzyme activity reconstitution.Communications biology · 2022Article
- Modulating the Coupling Efficiency of P450 BM3 by Controlling Water Diffusion through Access Tunnel Engineering.ChemSusChem · 2022Article
- Development of MEMS directed evolution strategy for multiplied throughput and convergent evolution of cytochrome P450 enzymes.Science China. Life sciences · 2022Article
- Cytochromes P450 in the biocatalytic valorization of lignin.Current opinion in biotechnology · 2022Review
- Engineering sequence and selectivity of late-stage C-H oxidation in the MycG iterative cytochrome P450.Journal of industrial microbiology & biotechnology · 2022Article
- De novo biosynthesis of bioactive isoflavonoids by engineered yeast cell factories.Nature communications · 2021Article
- Molecular Basis of Iterative C─H Oxidation by TamI, a Multifunctional P450 monooxygenase from the Tirandamycin Biosynthetic Pathway.ACS catalysis · 2020Article
- Computational-Based Mechanistic Study and Engineering of Cytochrome P450 MycG for Selective Oxidation of 16-Membered Macrolide Antibiotics.Journal of the American Chemical Society · 2020Article
- Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications.The Journal of biological chemistry · 2020Review
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Authors and funding
13 authors.
Funding
Abstract
Cytochrome P450 enzymes are capable of catalyzing a great variety of synthetically useful reactions such as selective C-H functionalization. Surrogate redox partners are widely used for reconstitution of P450 activity based on the assumption that the choice of these auxiliary proteins or their mode of action does not affect the type and selectivity of reactions catalyzed by P450s. Herein, we present an exceptional example to challenge this postulate. MycG, a multifunctional biosynthetic P450 monooxygenase responsible for hydroxylation and epoxidation of 16-membered ring macrolide mycinamicins, is shown to catalyze the unnatural N-demethylation(s) of a range of mycinamicin substrates when partnered with the free Rhodococcus reductase domain RhFRED or the engineered Rhodococcus-spinach hybrid reductase RhFRED-Fdx. By contrast, MycG fused with the RhFRED or RhFRED-Fdx reductase domain mediates only physiological oxidations. This finding highlights the larger potential role of variant redox partner protein-protein interactions in modulating the catalytic activity of P450 enzymes.
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