ArticlePloS one2014
Role of PKD2 in rheotaxis in Dictyostelium.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Ion Signaling in Cell Motility and Development inBiomolecules · 2024Review
- Emerging mechanistic understanding of cilia function in cellular signalling.Nature reviews. Molecular cell biology · 2024Review
- Polycystin-2 Mediated Calcium Signalling in theCells · 2024Article
- Membrane stretching activates calcium permeability of a putative channel Pkd2 during fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- Calcium responses to external mechanical stimuli in the multicellular stage of Dictyostelium discoideum.Scientific reports · 2022Article
- Fission yeast polycystin Pkd2p promotes cell size expansion and antagonizes the Hippo-related SIN pathway.Journal of cell science · 2022Article
- TheFrontiers in cell and developmental biology · 2022Article
- Article
- Origins of eukaryotic excitability.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Differential Roles of Actin Crosslinking Proteins Filamin and α-Actinin in Shear Flow-Induced Migration ofFrontiers in cell and developmental biology · 2021Article
- The Dynamics of Aerotaxis in a Simple Eukaryotic Model.Frontiers in cell and developmental biology · 2021Article
- Copine A regulates the size and exocytosis of contractile vacuoles and postlysosomes in Dictyostelium.FEBS open bio · 2020Article
- Article
- Extracellular signaling in Dictyostelium.The International journal of developmental biology · 2019Review
- A polycystin-type transient receptor potential (Trp) channel that is activated by ATP.Biology open · 2017Article
- Chemical and mechanical stimuli act on common signal transduction and cytoskeletal networks.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Inseparable tandem: evolution chooses ATP and Ca2+ to control life, death and cellular signalling.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Interplay between motility and cell-substratum adhesion in amoeboid cells.Biomicrofluidics · 2015Article
- Image based validation of dynamical models for cell reorientation.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sensing of mechanical forces modulates several cellular responses as adhesion, migration and differentiation. Transient elevations of calcium concentration play a key role in the activation of cells following mechanical stress, but it is still unclear how eukaryotic cells convert a mechanical signal into an ion flux. In this study, we used the model organism Dictyostelium discoideum to assess systematically the role of individual calcium channels in mechanosensing. Our results indicate that PKD2 is the major player in the cell response to rheotaxis (i.e., shear-flow induced mechanical motility), while other putative calcium channels play at most minor roles. Mutant pkd2 KO cells lose the ability to orient relative to a shear flow, whereas their ability to move towards a chemoattractant is unaffected. PKD2 is also important for calcium-induced lysosome exocytosis: WT cells show a transient, 2-fold increase in lysosome secretion upon sudden exposure to high levels of extracellular calcium, but pkd2 KO cells do not. In Dictyostelium, PKD2 is specifically localized at the plasma membrane, where it may generate calcium influxes in response to mechanical stress or extracellular calcium changes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.