Evidence map›Paper›PMID 24520401›Full record

ArticlePloS one2014

Evolution, three-dimensional model and localization of truncated hemoglobin PttTrHb of hybrid aspen.

Estelle Dumont, Soile Jokipii-Lukkari, Vimal Parkash, Jaana Vuosku, Robin Sundström, Yvonne Nymalm, Suvi Sutela, Katariina Taskinen, Pauli T Kallio, Tiina A Salminen and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Estelle DumontDepartment of Biology, University of Oulu, Oulu, Finland ; UMR-MD1, Transporteurs Membranaires, Chimiorésistance et Drug-Design, Aix-Marseille Université, Marseille, France.
Soile Jokipii-LukkariDepartment of Biology, University of Oulu, Oulu, Finland.
Vimal ParkashStructural Bioinformatics Laboratory, Department of Biosciences, Åbo Akademi University, Turku, Finland.
Jaana VuoskuDepartment of Biology, University of Oulu, Oulu, Finland.
Robin SundströmStructural Bioinformatics Laboratory, Department of Biosciences, Åbo Akademi University, Turku, Finland.
Yvonne NymalmStructural Bioinformatics Laboratory, Department of Biosciences, Åbo Akademi University, Turku, Finland.
Suvi SutelaDepartment of Biology, University of Oulu, Oulu, Finland.
Katariina TaskinenDepartment of Biology, University of Oulu, Oulu, Finland.
Pauli T KallioInstitute of Microbiology, ETH-Zürich, Zürich, Switzerland.
Tiina A SalminenStructural Bioinformatics Laboratory, Department of Biosciences, Åbo Akademi University, Turku, Finland.
Hely HäggmanDepartment of Biology, University of Oulu, Oulu, Finland.
University of Oulu · FIÅbo Akademi University · FIAix-Marseille Université · FRETH Zurich · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thus far, research on plant hemoglobins (Hbs) has mainly concentrated on symbiotic and non-symbiotic Hbs, and information on truncated Hbs (TrHbs) is scarce. The aim of this study was to examine the origin, structure and localization of the truncated Hb (PttTrHb) of hybrid aspen (Populus tremula L. × tremuloides Michx.), the model system of tree biology. Additionally, we studied the PttTrHb expression in relation to non-symbiotic class1 Hb gene (PttHb1) using RNAi-silenced hybrid aspen lines. Both the phylogenetic analysis and the three-dimensional (3D) model of PttTrHb supported the view that plant TrHbs evolved vertically from a bacterial TrHb. The 3D model suggested that PttTrHb adopts a 2-on-2 sandwich of α-helices and has a Bacillus subtilis -like ligand-binding pocket in which E11Gln and B10Tyr form hydrogen bonds to a ligand. However, due to differences in tunnel cavity and gate residue (E7Ala), it might not show similar ligand-binding kinetics as in Bs-HbO (E7Thr). The immunolocalization showed that PttTrHb protein was present in roots, stems as well as leaves of in vitro -grown hybrid aspens. In mature organs, PttTrHb was predominantly found in the vascular bundles and specifically at the site of lateral root formation, overlapping consistently with areas of nitric oxide (NO) production in plants. Furthermore, the NO donor sodium nitroprusside treatment increased the amount of PttTrHb in stems. The observed PttTrHb localization suggests that PttTrHb plays a role in the NO metabolism.

Indexed as

Biological EvolutionHybridization, GeneticModels, MolecularAmino Acid SequenceGene Expression Regulation, PlantHemeMolecular Sequence DataPhylogenyPlant LeavesPlants, Genetically ModifiedPlant StemsPopulusProtein TransportRNA InterferenceSequence AlignmentStructural Homology, ProteinHemeTruncated Hemoglobins

Identifiers

PMID24520401
PMCPMC3919811
OpenAlexW2050088209

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.