ArticlePloS one2014
Wall shear stress effects on endothelial-endothelial and endothelial-smooth muscle cell interactions in tissue engineered models of the vascular wall.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- In Silico Evaluation of Mechanobiological Parameters Under Variable Flow in Three-Dimensional Microfluidic Platforms Supporting Future Cell Migration Studies.Biomedicines · 2026Article
- Auxetic Stents as a Next Generation Solution for Vascular Diseases-A Review.Cardiovascular engineering and technology · 2026Review
- Wall shear stress: the challenges of a rising imaging marker in current clinical practice.Journal of thoracic disease · 2023Article
- Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress.Journal of vascular research · 2023Article
- Mechanical regulation of the early stages of angiogenesis.Journal of the Royal Society, Interface · 2022Review
- Human Vascular Wall Microfluidic Model for Preclinical Evaluation of Drug-Induced Vascular Injury.Tissue engineering. Part C, Methods · 2022Article
- Use of patient-specific computational models for optimization of aortic insufficiency after implantation of left ventricular assist device.The Journal of thoracic and cardiovascular surgery · 2021Article
- Patient-Specific Computational Fluid Dynamics Reveal Localized Flow Patterns Predictive of Post-Left Ventricular Assist Device Aortic Incompetence.Circulation. Heart failure · 2021Article
- Tissue engineered endometrial barrier exposed to peristaltic flow shear stresses.APL bioengineering · 2020Article
- Angiogenesis in Tissue Engineering: As Nature Intended?Frontiers in bioengineering and biotechnology · 2020Review
- Impact of Aortoseptal Angle Abnormalities and Discrete Subaortic Stenosis on Left-Ventricular Outflow Tract Hemodynamics: Preliminary Computational Assessment.Frontiers in bioengineering and biotechnology · 2020Article
- Impact of combined plaque structural stress and wall shear stress on coronary plaque progression, regression, and changes in composition.European heart journal · 2019Observational
- Shear Stress Attenuates Inward Remodeling in Cultured Mouse Thoracodorsal Arteries in an eNOS-Dependent, but Not Hemodynamic Manner, and Increases Cx37 Expression.Journal of vascular research · 2019Article
- Luteoloside Protects the Uterus from Staphylococcus aureus-Induced Inflammation, Apoptosis, and Injury.Inflammation · 2018Article
- Hemodynamics of cerebral bridging veins connecting the superior sagittal sinus based on numerical simulation.Biomedical engineering online · 2018Article
- Discrete Subaortic Stenosis: Perspective Roadmap to a Complex Disease.Frontiers in cardiovascular medicine · 2018Article
- Comparison of conventional and advanced in vitro models in the toxicity testing of nanoparticles.Artificial cells, nanomedicine, and biotechnology · 2018Review
- Phenotypic transformation of smooth muscle cells from porcine coronary arteries is associated with connexin 43.Molecular medicine reports · 2016Article
- Overexpression of YB1 C-terminal domain inhibits proliferation, angiogenesis and tumorigenicity in a SK-BR-3 breast cancer xenograft mouse model.FEBS open bio · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vascular functions are affected by wall shear stresses (WSS) applied on the endothelial cells (EC), as well as by the interactions of the EC with the adjacent smooth muscle cells (SMC). The present study was designed to investigate the effects of WSS on the endothelial interactions with its surroundings. For this purpose we developed and constructed two co-culture models of EC and SMC, and compared their response to that of a single monolayer of cultured EC. In one co-culture model the EC were cultured on the SMC, whereas in the other model the EC and SMC were cultured on the opposite sides of a membrane. We studied EC-matrix interactions through focal adhesion kinase morphology, EC-EC interactions through VE-Cadherin expression and morphology, and EC-SMC interactions through the expression of Cx43 and Cx37. In the absence of WSS the SMC presence reduced EC-EC connectivity but produced EC-SMC connections using both connexins. The exposure to WSS produced discontinuity in the EC-EC connections, with a weaker effect in the co-culture models. In the EC monolayer, WSS exposure (12 and 4 dyne/cm(2) for 30 min) increased the EC-EC interaction using both connexins. WSS exposure of 12 dyne/cm(2) did not affect the EC-SMC interactions, whereas WSS of 4 dyne/cm(2) elevated the amount of Cx43 and reduced the amount of Cx37, with a different magnitude between the models. The reduced endothelium connectivity suggests that the presence of SMC reduces the sealing properties of the endothelium, showing a more inflammatory phenotype while the distance between the two cell types reduced their interactions. These results demonstrate that EC-SMC interactions affect EC phenotype and change the EC response to WSS. Furthermore, the interactions formed between the EC and SMC demonstrate that the 1-side model can simulate better the arterioles, while the 2-side model provides better simulation of larger arteries.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.