ArticleExperimental and therapeutic medicine2014
Effects of BMP2 and VEGF165 on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells.
Article in Experimental and therapeutic medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- Osteogenic Differentiation in Chitosan-Based Scaffolds via P28 and VEGF Delivery.Molecules (Basel, Switzerland) · 2025Article
- Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds.Gels (Basel, Switzerland) · 2023Article
- The role of current herbal extracts in bone regeneration through dental implants:Archives of medical science : AMS · 2023Article
- Cytokine co-stimulation effect on odontogenic differentiation of stem cells.Clinical oral investigations · 2022Article
- MicroRNA treatment modulates osteogenic differentiation potential of mesenchymal stem cells derived from human chorion and placenta.Scientific reports · 2021Article
- The induced membrane technique for bone defects: Basic science, clinical evidence, and technical tips.OTA international : the open access journal of orthopaedic trauma · 2021Article
- Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats.European journal of histochemistry : EJH · 2020Article
- Combined Delivery of Two Different Bioactive Factors Incorporated in Hydroxyapatite Microcarrier for Bone Regeneration.Tissue engineering and regenerative medicine · 2020Article
- Adenoviral mediated mono delivery of BMP2 is superior to the combined delivery of BMP2 and VEGFA in bone regeneration in a critical-sized rat calvarial bone defect.Bone reports · 2019Article
- Stimulatory Effects of Boron Containing Bioactive Glass on Osteogenesis and Angiogenesis of Polycaprolactone: In Vitro Study.BioMed research international · 2019Article
- Synthesis and characterization of silibinin/phenanthroline/neocuproine copper(II) complexes for augmenting bone tissue regeneration: an in vitro analysis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- BMP2 and VEGF165 transfection to bone marrow stromal stem cells regulate osteogenic potential in vitro.Medicine · 2018Observational
- Delivery of VEGFA in bone marrow stromal cells seeded in copolymer scaffold enhances angiogenesis, but is inadequate for osteogenesis as compared with the dual delivery of VEGFA and BMP2 in a subcutaneous mouse model.Stem cell research & therapy · 2018Article
- Effect of Semelil, an Herbal Selenium-Based Medicine, on New Bone Formation in Calvarium of Rabbits.BioMed research international · 2018Article
- Growth factor sequestration and enzyme-mediated release from genipin-crosslinked gelatin microspheres.Journal of biomaterials science. Polymer edition · 2017Article
- Vertical bone regeneration using rhBMP-2 and VEGF.Head & face medicine · 2017Article
- The Effects of Epigallocatechin-3-Gallate and Mechanical Stimulation on Osteogenic Differentiation of Human Mesenchymal Stem Cells: Individual or Synergistic Effects.Tissue engineering and regenerative medicine · 2017Article
- Bone morphogenetic protein-2 enhances the osteogenic differentiation capacity of mesenchymal stromal cells derived from human bone marrow and umbilical cord.International journal of molecular medicine · 2017Article
- Proliferation and osteogenic differentiation of rat BMSCs on a novel Ti/SiC metal matrix nanocomposite modified by friction stir processing.Scientific reports · 2016Article
- Dickkopf-1 has an Inhibitory Effect on Mesenchymal Stem Cells to Fibroblast Differentiation.Chinese medical journal · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone marrow-derived mesenchymal stem cells (MSCs) are dominant seed cell sources for bone regeneration. Bone morphogenetic proteins (BMPs) initiate cartilage and bone formation in a sequential cascade. Vascular endothelial growth factor (VEGF) is an essential coordinator of extracellular matrix remodeling, angiogenesis and bone formation. In the present study, the effects of the vascular endothelial growth factor 165 (VEGF165) and bone morphogenetic protein 2 (BMP2) genes on bone regeneration were investigated by the lentivirus-mediated cotransfection of the two genes into rat bone marrow-derived MSCs. The successful co-expression of the two genes in the MSCs was confirmed using quantitative polymerase chain reaction (qPCR) and western blot analysis. The results of alizarin red and alkaline phosphatase (ALP) staining at 14 days subsequent to transfection showed that the area of staining in cells transfected with BMP2 alone was higher than that in cells transfected with BMP2 and VEGF165 or untransfected control cells, while the BMP2 + VEGF165 group showed significantly more staining than the untransfected control. This indicated that BMP2 alone exhibited a stronger effect in bone regeneration than BMP2 in combination with VEGF165. Similarly, in inducing culture medium, the ALP activity of the BMP2 + VEGF165 group was notably suppressed compared with that of the BMP2 group. The overexpression of VEGF165 inhibited BMP2-induced MSC differentiation and osteogenesis
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