ArticleRapid communications in mass spectrometry : RCM2014
Proteome degradation in fossils: investigating the longevity of protein survival in ancient bone.
Article in Rapid communications in mass spectrometry : RCM, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 2 of them syntheses that pooled it.
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63 citing papers in PubMed, 2 syntheses or guidelines pooled it, 190 citations in OpenAlex.
- Proteomic profiling of bone for the estimation of post-mortem interval and post-mortem submersion interval: a systematic review.International journal of legal medicine · 2026Pooled it
- Forensic applications of postmortem protein analysis in estimating the time since death: a systematic review.International journal of legal medicine · 2026Pooled it
- Collagen fingerprinting and sequence analysis provides a molecular phylogeny of extinct Australian megafauna.Proceedings. Biological sciences · 2025Article
- A library of avian proteins improves palaeoproteomic taxonomic identification and reveals widespread intraspecies variability.Nature communications · 2025Article
- Systematic Comparison of Bone Proteome Extraction Methods to Allow for Integrated Proteomics-Metabolomics Correlation.Journal of proteome research · 2025Article
- Advances in the Study of Protein Deamidation: Unveiling Its Influence on Aging, Disease Progression, Forensics and Therapeutic Efficacy.Proteomes · 2025Review
- Evidence for Endogenous Collagen inAnalytical chemistry · 2025Article
- Taphonomic variation in vascular remains from Mesozoic non-avian dinosaurs.Scientific reports · 2025Article
- Article
- Enhancing late postmortem interval prediction: a pilot study integrating proteomics and machine learning to distinguish human bone remains over 15 years.Biological research · 2024Article
- Nanoscopic imaging of ancient protein and vasculature offers insight into soft tissue and biomolecule fossilization.iScience · 2024Article
- Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen.iScience · 2024Article
- Bone of contention: Intra-element variability in remodelling of human femora based on histomorphometric and isotope analyses.PloS one · 2024Article
- A double-blind comparison of morphological and collagen fingerprinting (ZooMS) methods of skeletal identifications from Paleolithic contexts.Scientific reports · 2023Article
- Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus.Journal of proteome research · 2023Article
- Biomolecular histology as a novel proxy for ancient DNA and protein sequence preservation.Ecology and evolution · 2022Article
- Paleoproteomics.Chemical reviews · 2022Review
- Molecular signatures written in bone proteins of 79 AD victims from Herculaneum and Pompeii.Scientific reports · 2022Article
- SPIN enables high throughput species identification of archaeological bone by proteomics.Nature communications · 2022Article
- Revisiting proboscidean phylogeny and evolution through total evidence and palaeogenetic analyses includingiScience · 2022Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
rationaleWe report the use of proteomics techniques to study how the fossil bone proteome changes in complexity over one million years.
methodsWe include the attempted use of a previously unreported methodology in proteome research, to remove the dominant bone collagens using bacterial collagenase as well as conventional shotgun proteomics methodology following digestion with the protease trypsin. In this study we expand upon a set of 19 bovine sub-fossil specimens ranging over one and a half million years that had previously been shown to possess collagen, using a total of 46 LTQ-Orbitrap liquid chromatography/tandem mass spectrometry (LC/MS/MS) analyses containing 462,186 precursor ion analyses.
resultsAlthough many types of proteins can typically be identified in recent bone, in degraded bone we observe a rapid loss of lower abundance proteins. Abundant serum proteins such as serum albumin and alpha-2-HS-glycoprotein appear to be more easily recovered in ancient bone, both being identified in specimens dating to the Early Pleistocene, the earliest period tested in this study. Proteins belonging to the leucine-rich repeat family such as lumican, biglycan and chondroadherin also survive well, possibly because of their interactions with bone collagen.
conclusionsOf these 'survivor proteins' A2HSG shows a remarkable amount of sequence variation, making it potentially one of the most useful proteins to study for species identification and phylogenetic inference in archaeological and palaeontological bone.
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