Evidence map›Paper›PMID 24519735›Full record

ArticlePhytotherapy research : PTR2014

The isolation of Staphylococcus aureus tea tree oil-reduced susceptibility mutants.

Jesus A Cuaron, Santosh Dulal, Peter H Cooke, Nathanial J Torres, John E Gustafson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Phytotherapy research : PTR, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Jesus A CuaronMicrobiology Group, Department of Biology, New Mexico State University, Las Cruces, NM, 88003, USA.
Santosh Dulal
Peter H Cooke
Nathanial J Torres
John E Gustafson

Funding

NM-INBRE Sequencing and Bioinformatics CoreP20GM103451 · NIGMS · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI Charlotte C. Gard · 2012 to 2026
$61.2M
ZUNI HEALTH INITIATIVE-PILOT STUDYP20RR016480 · NCRR · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI ZHANG, PENG F · 2001 to 2011
$33.7M
RISE PROGRAM AT NEW MEXICO STATE UNIVERSITYR25GM061222 · NIGMS · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI HOUSTON, JESSICA PEREA, REDDI, LAKSHMI · 2000 to 2022
$21.3M
Undergraduate Biomedical Research Training at New Mexico State Univ., Las CrucesT34GM007667 · NIGMS · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI UNGUEZ, GRACIELA ALEXANDRA · 1985 to 2022
$13.6M
Genetic Determinants of the hVISA MechanismSC1AI081425 · NIAID · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI GUSTAFSON, JOHN E · 2008 to 2011
$1.2M
NCRR NIH HHS P20 RR016480NIAID NIH HHS SC1 AI081425NIGMS NIH HHS P20 GM103451NIGMS NIH HHS P20GM103451NIGMS NIH HHS R25 GM061222NIGMS NIH HHS R25 GM07667-30NIGMS NIH HHS S06-GM61222-05NIGMS NIH HHS SC1GM083882-01NIGMS NIH HHS T34 GM007667
6 · The paper itself

Abstract

Tea tree oil (TTO)-reduced susceptibility (TTORS) mutants of two Staphylococcus aureus laboratory strains were isolated utilizing TTO gradient plates. Attempts to isolate TTORS mutants employing agar plates containing single TTO concentrations failed. All TTORS mutants demonstrated a small colony variant (SCV) phenotype and produced cells with a smaller diameter, as determined by scanning electron microscopy. The addition of SCV auxotrophic supplements to media did not lead to an increase in TTORS mutant colony size. Revertants were also isolated from the TTORS mutants following growth in drug-free media, and all revertant strains demonstrated phenotypes similar to their respective parent strains. Transmission electron microscopy revealed that an SH1000 TTORS mutant demonstrated a thinner cell wall and novel septal invaginations compared with parent strain SH1000. In addition, comparative genomic sequencing did not reveal any mutations in an SH1000 TTORS mutant previously linked to well-characterized SCV genotypes. This study demonstrates that TTO can select for a unique SCV phenotype.

Indexed as

Culture MediaDNA Mutational AnalysisGenotypeMicrobial Sensitivity TestsMicroscopy, Electron, ScanningMicroscopy, Electron, TransmissionMutationPhenotypeStaphylococcus aureusTea Tree OilCulture MediaTea Tree Oilelectron microscopygenomicsreduced susceptibilitysmall colony variantsStaphylococcus aureustea tree oil

Identifiers

PMID24519735
PMCPMC4125540

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.