ArticleInvestigative ophthalmology & visual science2014
Localization of Cacna1s to ON bipolar dendritic tips requires mGluR6-related cascade elements.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Glutamate transporters are involved in direct inhibitory synaptic transmission in the vertebrate retina.Open biology · 2024Article
- Multiple Calcium Channel Types with Unique Expression Patterns Mediate Retinal Signaling at Bipolar Cell Ribbon Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Skeletal muscle CaPflugers Archiv : European journal of physiology · 2020Review
- Development of cone photoreceptors and their synapses in the human and monkey fovea.The Journal of comparative neurology · 2019Article
- A group I metabotropic glutamate receptor controls synaptic gain between rods and rod bipolar cells in the mouse retina.Physiological reports · 2018Article
- Relevance of tissue specific subunit expression in channelopathies.Neuropharmacology · 2018Review
- iPSC-Derived Retina Transplants Improve Vision in rd1 End-Stage Retinal-Degeneration Mice.Stem cell reports · 2017Article
- Lack of mGluR6-related cascade elements leads to retrograde trans-synaptic effects on rod photoreceptor synapses via matrix-associated proteins.The European journal of neuroscience · 2016Article
- CACNA1S expression in mouse retina: Novel isoforms and antibody cross-reactivity with GPR179.Visual neuroscience · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeL-type voltage gated calcium channels in retina localize primarily at the presynaptic active zones of photoreceptors and bipolar cells where they modulate glutamate release. However, the pore forming subunit Cacna1s of certain L-type channels is also expressed postsynaptically at the tips of ON bipolar cell dendrites where it colocalizes with mGluR6, but has an unknown function. At these dendritic tips, the components of the mGluR6 signaling cascade cluster together in a macromolecular complex, and each one's localization often depends on that of the others. Thus, we explored if Cacna1s is part of the mGluR6 complex.
methodsWe determined Cacna1s expression by PCR using an ON bipolar library, by Western blotting, and by standard immunohistochemistry.
resultsThe PCR amplification confirmed expression of the transcript in ON bipolar cells, and Western blotting showed the expected bands. Immunostaining for Cacna1s was stronger in the dendritic tips of rod bipolar cells than in those of ON cone bipolar cells. This staining severely decreased in mice missing various mGluR6 cascade elements (Grm6(-/-), Gnao1(-/-), Gnb3(-/-), Gng13(-/-), and Trpm1(-/-)). During development, the ratio of the number of Cacna1s puncta to the number of presynaptic ribbons followed a sigmoidal pattern, rising rapidly from P13 to P17. The mGluR6 expression preceded that of Cacna1s and RGS11.
conclusionsOur results show that the localization and stability of Cacna1s depend on the expression of mGluR6 and its cascade components, and they suggest that Cacna1s is part of the mGluR6 complex. We hypothesize that Cacna1s contributes to light adaptation by permeating calcium.
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