Chondrogenic Differentiation of Human Umbilical Cord Blood-Derived Unrestricted Somatic Stem Cells on A 3D Beta-Tricalcium Phosphate-Alginate-Gelatin Scaffold.

Masoud Soleimani, Layasadat Khorsandi, Amir Atashi, Fereshteh Nejaddehbashi

Abstract read

In one paragraph

Article in Cell journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it

0cells of the map it votes in

7citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

Ameliorating effect of encapsulated hepatocyte-like cells derived from umbilical cord in high mannuronic alginate scaffolds on acute liver failure in rats.Iranian journal of basic medical sciences · 2018
Article
Effects of Silymarin-Loaded Nanoparticles on HT-29 Human Colon Cancer Cells.Medicina (Kaunas, Lithuania) · 2018
Article
Hypoxia Is a Critical Parameter for Chondrogenic Differentiation of Human Umbilical Cord Blood Mesenchymal Stem Cells in Type I/III Collagen Sponges.International journal of molecular sciences · 2017
Article
Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Contribute to Chondrogenesis in Coculture with Chondrocytes.BioMed research international · 2016
Article
Manipulation of a quasi-natural cell block for high-efficiency transplantation of adherent somatic cells.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2015
Article
Human Menstrual Blood-Derived Endometrial Stem Cells Promote Functional Recovery by Improving the Inflammatory Microenvironment in a Mouse Spinal Cord Injury Model.Cell transplantation
Article
Biomechanical analysis of mandibular cartilage defect regeneration after implantation of human umbilical cord mesenchymal stem cells and a platelet rich fibrin scaffold: an experimental animal study.Journal of oral biology and craniofacial research
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Masoud SoleimaniDepartment of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Layasadat KhorsandiCellular and Molecular Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Amir AtashiDepartment of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Fereshteh NejaddehbashiDepartment of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. and Department of Cell Biology, Khatam University, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveFinding cell sources for cartilage tissue engineering is a critical procedure. The purpose of the present experimental study was to test the in vitro efficacy of the beta-tricalcium phosphate-alginate-gelatin (BTAG) scaffold to induce chondrogenic differentiation of human umbilical cord blood-derived unrestricted somatic stem cells (USSCs). MATERIALS AND

methodsIn this experimental study, USSCs were encapsulated in BTAG scaffold and cultured for 3 weeks in chondrogenic medium as chondrogenic group and in Dulbecco's Modified Eagle's Medium (DMEM) as control group. Chondrogenic differentiation was evaluated by histology, immunofluorescence and RNA analyses for the expression of cartilage extracellular matrix components. The obtain data were analyzed using SPSS version 15.

resultsHistological and immunohistochemical staining revealed that collagen II was markedly expressed in the extracellular matrix of the seeded cells on scaffold in presence of chondrogenic media after 21 days. Reverse transcription-polymerase chain reaction (RT-PCR) showed a significant increase in expression levels of genes encoded the cartilage-specific markers, aggrecan, type I and II collagen, and bone morphogenetic protein (BMP)-6 in chondrogenic group.

conclusionThis study demonstrates that BTAG can be considered as a suitable scaffold for encapsulation and chondrogenesis of USSCs.

Indexed as

ChondrogenesisMesenchymal Stem CellsScaffold

Identifiers

PMID24518974

PMCPMC3933438

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