Evidence map›Paper›PMID 24518856›Full record

ArticleScientific reports2014

Short RNA indicator sequences are not completely degraded by autoclaving.

Veena V Unnithan, Adrian Unc, Valerisa Joe, Geoffrey B Smith

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Veena V UnnithanDepartment of Biology, New Mexico State University, Las Cruces, NM, USA, 88003.
Adrian Unc1] Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM, USA, 88003 [2] Memorial University of Newfoundland, Boreal Ecosystems Research Initiative, Corner Brook, NL, Canada, A2H 6P9.
Valerisa JoeDepartment of Biology, New Mexico State University, Las Cruces, NM, USA, 88003.
Geoffrey B SmithDepartment of Biology, New Mexico State University, Las Cruces, NM, USA, 88003.

Funding

RISE PROGRAM AT NEW MEXICO STATE UNIVERSITYR25GM061222 · NIGMS · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI HOUSTON, JESSICA PEREA, REDDI, LAKSHMI · 2000 to 2022
$21.3M
NIGMS NIH HHS R25 GM061222NIGMS NIH HHS R25GM061222
6 · The paper itself

Abstract

Short indicator RNA sequences (<100 bp) persist after autoclaving and are recovered intact by molecular amplification. Primers targeting longer sequences are most likely to produce false positives due to amplification errors easily verified by melting curves analyses. If short indicator RNA sequences are used for virus identification and quantification then post autoclave RNA degradation methodology should be employed, which may include further autoclaving.

Indexed as

Real-Time Polymerase Chain ReactionSterilizationBase SequenceDNA PrimersHot TemperatureLevivirusRNA, ViralDNA PrimersRNA, Viral

Identifiers

PMID24518856
PMCPMC3921633

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.