ArticleVirulence2014
Biofilm-degrading enzymes from Lysobacter gummosus.
Article in Virulence, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Predatory bacteria as potential biofilm control and eradication agents in the food industry.Food science and biotechnology · 2023Review
- Comparative genomics provides insights into the potential biocontrol mechanism of twoFrontiers in microbiology · 2022Article
- Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis.Biotechnology for biofuels · 2021Article
- Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using ExperimentalFrontiers in bioengineering and biotechnology · 2021Article
- Lytic potential of Lysobacter capsici VKM B-2533Scientific reports · 2020Article
- Phage-Derived Antibacterials: Harnessing the Simplicity, Plasticity, and Diversity of Phages.Viruses · 2019Review
- Differential effects of coral-giant clam assemblages on biofouling formation.Scientific reports · 2019Article
- Structural and functional properties of antimicrobial protein L5 of Lysоbacter sp. XL1.Applied microbiology and biotechnology · 2018Article
- The Lysobacter capsici AZ78 Genome Has a Gene Pool Enabling it to Interact Successfully with Phytopathogenic Microorganisms and Environmental Factors.Frontiers in microbiology · 2016Article
- Comparative genomics and metabolic profiling of the genus Lysobacter.BMC genomics · 2015Article
- Diversity and Activity of Lysobacter Species from Disease Suppressive Soils.Frontiers in microbiology · 2015Article
- Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilm-degrading enzymes could be used for the gentle cleaning of industrial and medical devices and the manufacture of biofilm-resistant materials. We therefore investigated 20 species and strains of the bacterial genus Lysobacter for their ability to degrade experimental biofilms formed by Staphylococcus epidermidis, a common nosocomial pathogen typically associated with device-related infections. The highest biofilm-degradation activity was achieved by L. gummosus. The corresponding enzymes were identified by sequencing the L. gummosus genome. Partial purification of the biofilm-degrading activity from an extract of extracellular material followed by peptide mass fingerprinting resulted in the identification of two peptidases (α-lytic protease and β-lytic metalloendopeptidase) that were predicted to degrade bacterial cell walls. In addition, we identified two isoforms of a lysyl endopeptidase and an enzyme similar to metalloproteases from Vibrio spp. Potential peptidoglycan-binding C-terminal fragments of two OmpA-like proteins also co-purified with the biofilm-degrading activity. The L. gummosus genome was found to encode five isoenzymes of α-lytic protease and three isoenzymes of lysyl endopeptidase. These results indicated that the extracellular digestion of biofilms by L. gummosus depends on multiple bacteriolytic and proteolytic enzymes, which could now be exploited for biofilm control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.