ArticleArthritis research & therapy2013
Mast cells exert pro-inflammatory effects of relevance to the pathophyisology of tendinopathy.
Article in Arthritis research & therapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- A systematic review of inflammatory cells and markers in human tendinopathy.BMC musculoskeletal disorders · 2020Pooled it
- Different Achilles Tendon Pathologies Show Distinct Histological and Molecular Characteristics.International journal of molecular sciences · 2018Trial
- Adelmidrol plus hyaluronic acid injections in the management of shoulder impairment due to rotator cuff disease: an exploratory pilot observational retrospective investigation.Musculoskeletal surgery · 2026Article
- Pathological Mechanisms of Tendinopathy Pain: From Mechanical Overload to Neuroimmune-Vascular Crosstalk.Pain research & management · 2026Review
- Review
- Is Chronic Tendon Pain Caused by Neuropathy? Exciting Breakthroughs may Direct Potential Treatment.Current pain and headache reports · 2024Review
- MRGPRX2-mediated mast cell activation by substance P from overloaded human tenocytes induces inflammatory and degenerative responses in tendons.Scientific reports · 2024Article
- TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.Nature communications · 2023Article
- The application of mechanical load onto mouse tendons by magnetic restraining represses Mmp-3 expression.BMC research notes · 2023Article
- Immune mechanisms in vulvodynia: key roles for mast cells and fibroblasts.Frontiers in cellular and infection microbiology · 2023Review
- Innate and adaptive immune system cells implicated in tendon healing and disease.European cells & materials · 2022Review
- The Alleviating Effect ofMolecules (Basel, Switzerland) · 2022Article
- Review
- Interplay of Forces and the Immune Response for Functional Tendon Regeneration.Frontiers in cell and developmental biology · 2021Review
- MRGPRX2 and Adverse Drug Reactions.Frontiers in immunology · 2021Review
- Human Tendon Stem/Progenitor Cell Features and Functionality Are Highly Influenced byFrontiers in bioengineering and biotechnology · 2021Article
- Molecular and Structural Effects of Percutaneous Interventions in Chronic Achilles Tendinopathy.International journal of molecular sciences · 2020Review
- A Perspective on Reversibility of Tendinosis-Induced Multi-Level Adaptations.Frontiers in physiology · 2020Article
- MicroRNA29a Treatment Improves Early Tendon Injury.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Teriparatide Treatment Improves Bone Defect Healing Via Anabolic Effects on New Bone Formation and Non-Anabolic Effects on Inhibition of Mast Cells in a Murine Cranial Window Model.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
introductionWe have previously found an increased mast cell density in tendon biopsies from patients with patellar tendinopathy compared to controls. This study examined the influence of mast cells on basic tenocyte functions, including production of the inflammatory mediator prostaglandin E2 (PGE2), extracellular matrix remodeling and matrix metalloproteinase (MMP) gene transcription, and collagen synthesis.
methodsPrimary human tenocytes were stimulated with an established human mast cell line (HMC-1). Extracellular matrix remodeling was studied by culturing tenocytes in a three-dimensional collagen lattice. Survival/proliferation was assessed with the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) assay. Levels of mRNA for COX-2, COL1A1, MMP1, and MMP7 were determined by quantitative real-time polymerase chain reaction (qPCR). Cox-2 protein level was assessed by Western blot analysis and type I procollagen was detected by immunofluorescent staining. PGE2 levels were determined using an enzyme-linked immunosorbent assay (ELISA).
resultsMast cells stimulated tenocytes to produce increased levels of COX-2 and the pro-inflammatory mediator PGE2, which in turn decreased COL1A1 mRNA expression. Additionally, mast cells reduced the type I procollagen protein levels produced by tenocytes. Transforming growth factor beta 1 (TGF-β1) was responsible for the induction of Cox-2 and PGE2 by tenocytes. Mast cells increased MMP1 and MMP7 transcription and increased the contraction of a three-dimensional collagen lattice by tenocytes, a phenomenon which was blocked by a pan-MMP inhibitor (Batimastat).
conclusionOur data demonstrate that mast cell-derived PGE2 reduces collagen synthesis and enhances expression and activities of MMPs in human tenocytes.
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Registered trials
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