ArticleParasites & vectors2013
Rapid detection of Echinococcus species by a high-resolution melting (HRM) approach.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis.Parasites & vectors · 2022Article
- Article
- Development of High Resolution Melting Analysis as a Diagnostic Tool for Molecular Detection ofIranian journal of public health · 2020Article
- Functional characterization of the translation initiation factor eIF4E of Echinococcus granulosus.Parasitology research · 2019Article
- Is species identification of Echinostoma revolutum using mitochondrial DNA barcoding feasible with high-resolution melting analysis?Parasitology research · 2019Article
- A novel PCR-RFLP assay for molecular characterization of Echinococcus granulosus sensu lato and closely related species in developing countries.Parasitology research · 2016Article
- High-resolution melting analysis (HRM) for differentiation of four major Taeniidae species in dogs Taenia hydatigena, Taenia multiceps, Taenia ovis, and Echinococcus granulosus sensu stricto.Parasitology research · 2016Article
- Identification of gene mutation in patients with osteogenesis imperfect using high resolution melting analysis.Scientific reports · 2015Article
- Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis.International journal of molecular sciences · 2015Article
- Validation of suitable reference genes for expression normalization in Echinococcus spp. larval stages.PloS one · 2014Article
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5 authors.
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Abstract
backgroundHigh-resolution melting (HRM) provides a low-cost, fast and sensitive scanning method that allows the detection of DNA sequence variations in a single step, which makes it appropriate for application in parasite identification and genotyping. The aim of this work was to implement an HRM-PCR assay targeting part of the mitochondrial cox1 gene to achieve an accurate and fast method for Echinococcus spp. differentiation.
findingsFor melting analysis, a total of 107 samples from seven species were used in this study. The species analyzed included Echinococcus granulosus (n = 41) and Echinococcus ortleppi (n = 50) from bovine, Echinococcus vogeli (n = 2) from paca, Echinococcus oligarthra (n = 3) from agouti, Echinococcus multilocularis (n = 6) from monkey and Echinococcus canadensis (n = 2) and Taenia hydatigena (n = 3) from pig. DNA extraction was performed, and a 444-bp fragment of the cox1 gene was amplified. Two approaches were used, one based on HRM analysis, and a second using SYBR Green Tm-based. In the HRM analysis, a specific profile for each species was observed. Although some species exhibited almost the same melting temperature (Tm) value, the HRM profiles could be clearly discriminated. The SYBR Green Tm-based analysis showed differences between E. granulosus and E. ortleppi and between E. vogeli and E. oligarthra.
conclusionsIn this work, we report the implementation of HRM analysis to differentiate species of the genus Echinococcus using part of the mitochondrial gene cox1. This method may be also potentially applied to identify other species belonging to the Taeniidae family.
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