ArticlePloS one2014
Growth factor priming differentially modulates components of the extracellular matrix proteome in chondrocytes and synovium-derived stem cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- The checkpoint inhibitor PD-1H/VISTA controls osteoclast-mediated multiple myeloma bone disease.Nature communications · 2023Article
- Toward defining the role of the synovium in mitigating normal articular cartilage wear and tear.Journal of biomechanics · 2023Article
- Changes in extracellular matrix in failing human non-ischemic and ischemic hearts with mechanical unloading.Journal of molecular and cellular cardiology · 2022Article
- Biophysical Modulation of Mesenchymal Stem Cell Differentiation in the Context of Skeletal Repair.International journal of molecular sciences · 2022Review
- Review
- Pulsed electromagnetic fields promote repair of focal articular cartilage defects with engineered osteochondral constructs.Biotechnology and bioengineering · 2020Article
- Cartilage Wear Particles Induce an Inflammatory Response Similar to Cytokines in Human Fibroblast-Like Synoviocytes.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- A Functional Tissue-Engineered Synovium Model to Study Osteoarthritis Progression and Treatment.Tissue engineering. Part A · 2019Article
- Identifying the Growth Factors for Improving Neointestinal Regeneration in Rats through Transcriptome Analysis Using RNA-Seq Data.BioMed research international · 2018Article
- Transient expression of the diseased phenotype of osteoarthritic chondrocytes in engineered cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Article
- Concise Review: Mesenchymal Stem Cells for Functional Cartilage Tissue Engineering: Taking Cues from Chondrocyte-Based Constructs.Stem cells translational medicine · 2017Review
- Environmental preconditioning rejuvenates adult stem cells' proliferation and chondrogenic potential.Biomaterials · 2017Review
- Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis.Nature chemical biology · 2016Article
- High seeding density of human chondrocytes in agarose produces tissue-engineered cartilage approaching native mechanical and biochemical properties.Journal of biomechanics · 2016Article
- In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts.In vitro cellular & developmental biology. Animal · 2016Article
- Passage-dependent relationship between mesenchymal stem cell mobilization and chondrogenic potential.Osteoarthritis and cartilage · 2015Article
- Platelet-rich plasma releasate differently stimulates cellular commitment toward the chondrogenic lineage according to concentration.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
To make progress in cartilage repair it is essential to optimize protocols for two-dimensional cell expansion. Chondrocytes and SDSCs are promising cell sources for cartilage repair. We previously observed that priming with a specific growth factor cocktail (1 ng/mL transforming growth factor-β1, 5 ng/mL basic fibroblast growth factor, and 10 ng/mL platelet-derived growth factor-BB) in two-dimensional culture, led to significant improvement in mechanical and biochemical properties of synovium-derived stem cell (SDSC)-seeded constructs. The current study assessed the effect of growth factor priming on the proteome of canine chondrocytes and SDSCs. In particular, growth factor priming modulated the proteins associated with the extracellular matrix in two-dimensional cultures of chondrocytes and SDSCs, inducing a partial dedifferentiation of chondrocytes (most proteins associated with cartilage were down-regulated in primed chondrocytes) and a partial differentiation of SDSCs (some collagen-related proteins were up-regulated in primed SDSCs). However, when chondrocytes and SDSCs were grown in pellet culture, growth factor-primed cells maintained their chondrogenic potential with respect to glycosaminoglycan and collagen production. In conclusion, the strength of the label-free proteomics technique is that it allows for the determination of changes in components of the extracellular matrix proteome in chondrocytes and SDSCs in response to growth factor priming, which could help in future tissue engineering strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.