ArticlePloS one2014
Neural circuit interactions between the dorsal raphe nucleus and the lateral hypothalamus: an experimental and computational study.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Impact of Circadian Rhythm and Sleep Architecture Changes on Prognosis in Patients with Acute Hemorrhagic Stroke.Nature and science of sleep · 2025Article
- Interactions between Lateral Hypothalamic Orexin and Dorsal Raphe Circuitry in Energy Balance.Brain sciences · 2024Article
- Degeneracy and stability in neural circuits of dopamine and serotonin neuromodulators: A theoretical consideration.Frontiers in computational neuroscience · 2022Article
- Serotonin, food intake, and obesity.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2021Review
- Opportunities for multiscale computational modelling of serotonergic drug effects in Alzheimer's disease.Neuropharmacology · 2020Review
- Sleep Modelling across Physiological Levels.Clocks & sleep · 2019Review
- Orexins, Sleep, and Blood Pressure.Current hypertension reports · 2018Review
- Chronic light exposure alters serotonergic and orexinergic systems in the rat brain and reverses maternal separation-induced increase in orexin receptors in the prefrontal cortex.Metabolic brain disease · 2018Article
- Toward a multiscale modeling framework for understanding serotonergic function.Journal of psychopharmacology (Oxford, England) · 2017Review
- An integrated modelling framework for neural circuits with multiple neuromodulators.Journal of the Royal Society, Interface · 2017Article
- Regulation of motivation for food by neuromedin U in the paraventricular nucleus and the dorsal raphe nucleus.International journal of obesity (2005) · 2017Article
- Integrated dopaminergic neuronal model with reduced intracellular processes and inhibitory autoreceptors.IET systems biology · 2015Article
- Neural correlates of consciousness: what we know and what we have to learn!Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2015Review
- Intrinsic and extrinsic cues regulate the daily profile of mouse lateral habenula neuronal activity.The Journal of physiology · 2014Article
- Interacting Neural Processes of Feeding, Hyperactivity, Stress, Reward, and the Utility of the Activity-Based Anorexia Model of Anorexia Nervosa.Harvard review of psychiatryReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Orexinergic/hypocretinergic (Ox) neurotransmission plays an important role in regulating sleep, as well as in anxiety and depression, for which the serotonergic (5-HT) system is also involved in. However, little is known regarding the direct and indirect interactions between 5-HT in the dorsal raphe nucleus (DRN) and Ox neurons in the lateral hypothalamus (LHA). In this study, we report the additional presence of 5-HT1BR, 5-HT2AR, 5-HT2CR and fast ligand-gated 5-HT3AR subtypes on the Ox neurons of transgenic Ox-enhanced green fluorescent protein (Ox-EGFP) and wild type C57Bl/6 mice using single and double immunofluorescence (IF) staining, respectively, and quantify the colocalization for each 5-HT receptor subtype. We further reveal the presence of 5-HT3AR and 5-HT1AR on GABAergic neurons in LHA. We also identify NMDAR1, OX1R and OX2R on Ox neurons, but none on adjacent GABAergic neurons. This suggests a one-way relationship between LHA's GABAergic and Ox neurons, wherein GABAergic neurons exerts an inhibitory effect on Ox neurons under partial DRN's 5-HT control. We also show that Ox axonal projections receive glutamatergic (PSD-95 immunopositive) and GABAergic (Gephyrin immunopositive) inputs in the DRN. We consider these and other available findings into our computational model to explore possible effects of neural circuit connection types and timescales on the DRN-LHA system's dynamics. We find that if the connections from 5-HT to LHA's GABAergic neurons are weakly excitatory or inhibitory, the network exhibits slow oscillations; not observed when the connection is strongly excitatory. Furthermore, if Ox directly excites 5-HT neurons at a fast timescale, phasic Ox activation can lead to an increase in 5-HT activity; no significant effect with slower timescale. Overall, our experimental and computational approaches provide insights towards a more complete understanding of the complex relationship between 5-HT in the DRN and Ox in the LHA.
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