ArticlePloS one2014
Gene expression profiling and secretome analysis differentiate adult-derived human liver stem/progenitor cells and human hepatic stellate cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
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- Uncovering a Novel Functional Interaction Between Adult Hepatic Progenitor Cells, Inflammation and EGFR Signaling During Bile Acids-Induced Injury.International journal of biological sciences · 2024Article
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- Biodegradable biliary stents coated with mesenchymal stromal cells in a porcine choledochojejunostomy model.Cytotherapy · 2023Article
- Adipose-derived stem cells in the treatment of hepatobiliary diseases and sepsis.World journal of clinical cases · 2022Review
- Human Liver Stem Cell Transplantation Alleviates Liver Fibrosis in a Rat Model of CClInternational journal of stem cells · 2021Article
- A Bioinformatic Analysis of Correlations between Polymeric Immunoglobulin Receptor (PIGR) and Liver Fibrosis Progression.BioMed research international · 2021Article
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- Adipose-Derived Stem Cells: Current Applications and Future Directions in the Regeneration of Multiple Tissues.Stem cells international · 2020Review
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- Human umbilical cord mesenchymal stem cells ameliorate liver fibrosisWorld journal of stem cells · 2019Review
- Rescue of degenerating neurons and cells by stem cell released molecules: using a physiological renormalization strategy.Physiological reports · 2019Article
- Combatting Fibrosis: Exosome-Based Therapies in the Regression of Liver Fibrosis.Hepatology communications · 2019Review
- Human Hepatocytes and Differentiated Adult-Derived Human Liver Stem/Progenitor Cells DisplayJournal of immunology research · 2019Article
- Cytokinome of adult-derived human liver stem/progenitor cells: immunological and inflammatory features.Hepatobiliary surgery and nutrition · 2018Article
- Human umbilical cord mesenchymal stem cells inhibit proliferation of hepatic stellate cells in vitro.International journal of molecular medicine · 2018Article
- An Elf2-like transcription factor acts as repressor of the mouse ecto-5'-nucleotidase gene expression in hepatic myofibroblasts.Purinergic signalling · 2017Article
- Comparison of bone marrow-vs. adipose tissue-derived mesenchymal stem cells for attenuating liver fibrosis.Experimental and therapeutic medicine · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adult-derived human liver stem/progenitor cells (ADHLSC) are obtained after primary culture of the liver parenchymal fraction. The cells are of fibroblastic morphology and exhibit a hepato-mesenchymal phenotype. Hepatic stellate cells (HSC) derived from the liver non-parenchymal fraction, present a comparable morphology as ADHLSC. Because both ADHLSC and HSC are described as liver stem/progenitor cells, we strived to extensively compare both cell populations at different levels and to propose tools demonstrating their singularity. ADHLSC and HSC were isolated from the liver of four different donors, expanded in vitro and followed from passage 5 until passage 11. Cell characterization was performed using immunocytochemistry, western blotting, flow cytometry, and gene microarray analyses. The secretion profile of the cells was evaluated using Elisa and multiplex Luminex assays. Both cell types expressed α-smooth muscle actin, vimentin, fibronectin, CD73 and CD90 in accordance with their mesenchymal origin. Microarray analysis revealed significant differences in gene expression profiles. HSC present high expression levels of neuronal markers as well as cytokeratins. Such differences were confirmed using immunocytochemistry and western blotting assays. Furthermore, both cell types displayed distinct secretion profiles as ADHLSC highly secreted cytokines of therapeutic and immuno-modulatory importance, like HGF, interferon-γ and IL-10. Our study demonstrates that ADHLSC and HSC are distinct liver fibroblastic cell populations exhibiting significant different expression and secretion profiles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.