ArticlePLoS pathogens2014
Lundep, a sand fly salivary endonuclease increases Leishmania parasite survival in neutrophils and inhibits XIIa contact activation in human plasma.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 103 citations in OpenAlex.
- Leishmaniasis in deployed military populations: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2025Pooled it
- New insights on the Feeding Site and Salivation by Lutzomyia longipalpis (Diptera: Psychodidae) During Blood Ingestion on Host Skin.PLoS neglected tropical diseases · 2026Article
- Genomic instability and mono-parental expression mitigate genomic shock in a cross-subgenusNAR molecular medicine · 2026Article
- Hematological Ratios inAnimals : an open access journal from MDPI · 2026Article
- Sand fly gut microbiota in Leishmania transmission: roles, interactions, and paratransgenic prospects.Archives of microbiology · 2025Review
- Cross-subgenus hybridization betweenbioRxiv : the preprint server for biology · 2025Article
- Functional plasticity shapes neutrophil response to Leishmania major infection in susceptible and resistant strains of mice.PLoS pathogens · 2024Article
- Neutrophil extracellular traps formation: effect of Leishmania major promastigotes and salivary gland homogenates of Phlebotomus papatasi in human neutrophil culture.BMC microbiology · 2024Article
- First evidence of experimental genetic hybridization between cutaneous and visceral strains of Leishmania donovani within its natural vector Phlebotomus argentipes.Acta tropica · 2023Article
- Balancing the functions of DNA extracellular traps in intracellular parasite infections: implications for host defense, disease pathology and therapy.Cell death & disease · 2023Review
- Article
- Is There Any Difference in the In Situ Immune Response in Active Localized Cutaneous Leishmaniasis That Respond Well or Poorly to Meglumine Antimoniate Treatment or Spontaneously Heal?Microorganisms · 2023Article
- An insight into the female and male Sabethes cyaneus mosquito salivary glands transcriptome.Insect biochemistry and molecular biology · 2023Article
- Exploring the leishmanicidal potential of terpenoids: a comprehensive review on mechanisms of cell death.Frontiers in cellular and infection microbiology · 2023Review
- Self-Hybridization in Leishmania major.mBio · 2022Article
- An overview of the sand fly salivary proteins in vaccine development against leishmaniases.Iranian journal of microbiology · 2022Review
- Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods.Parasites & vectors · 2022Review
- Sand flies: Basic information on the vectors of leishmaniasis and their interactions with Leishmania parasites.Communications biology · 2022Review
- Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunity.PLoS neglected tropical diseases · 2022Article
- Stress conditions promoteeLife · 2022Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Neutrophils are the host's first line of defense against infections, and their extracellular traps (NET) were recently shown to kill Leishmania parasites. Here we report a NET-destroying molecule (Lundep) from the salivary glands of Lutzomyia longipalpis. Previous analysis of the sialotranscriptome of Lu. longipalpis showed the potential presence of an endonuclease. Indeed, not only was the cloned cDNA (Lundep) shown to encode a highly active ss- and dsDNAse, but also the same activity was demonstrated to be secreted by salivary glands of female Lu. longipalpis. Lundep hydrolyzes both ss- and dsDNA with little sequence specificity with a calculated DNase activity of 300000 Kunitz units per mg of protein. Disruption of PMA (phorbol 12 myristate 13 acetate)- or parasite-induced NETs by treatment with recombinant Lundep or salivary gland homogenates increases parasite survival in neutrophils. Furthermore, co-injection of recombinant Lundep with metacyclic promastigotes significantly exacerbates Leishmania infection in mice when compared with PBS alone or inactive (mutagenized) Lundep. We hypothesize that Lundep helps the parasite to establish an infection by allowing it to escape from the leishmanicidal activity of NETs early after inoculation. Lundep may also assist blood meal intake by lowering the local viscosity caused by the release of host DNA and as an anticoagulant by inhibiting the intrinsic pathway of coagulation.
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Registered trials
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