ArticleThe Korean journal of parasitology2013
Molecular differentiation of Opisthorchis viverrini and Clonorchis sinensis eggs by multiplex real-time PCR with high resolution melting analysis.
Article in The Korean journal of parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 20 citations in OpenAlex.
- Current Prevalence of Infection with Opisthorchis viverrini and Intestinal Parasites in Rural Communities in Northeast Thailand Using Stool Examinations and Serological Screening Methods.The American journal of tropical medicine and hygiene · 2025Article
- Multiparasitism in Spain in a Korean Visiting Friends and Relatives: Case Report and Review of Imported Cases ofClinical case reports · 2025Article
- Study of the population genetic structure of Opisthorchis-like eggs in northern Thailand using mitochondrial genes.PLoS neglected tropical diseases · 2024Article
- Diagnosis of Human Trematode Infections.Advances in experimental medicine and biology · 2024Review
- Fish and Food-Fatale: Food-borne TrematodeHelminthologia · 2023Review
- Are Melanoides tuberculata and Tarebia granifera (Gastropoda, Thiaridae), suitable first intermediate hosts of Clonorchis sinensis in Vietnam?PLoS neglected tropical diseases · 2021Article
- Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics.PLoS neglected tropical diseases · 2020Article
- Development of Conventional Multiplex PCR: A Rapid Technique for Simultaneous Detection of Soil-Transmitted Helminths.Pathogens (Basel, Switzerland) · 2019Article
- Clonorchiasis sinensis detected by laparoscopic exploration of biliary tracts in two patients with obstructive jaundice.BMC infectious diseases · 2019Article
- Performance evaluation of existing immunoassays for Clonorchis sinensis infection in China.Parasites & vectors · 2018Article
- Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand.PLoS neglected tropical diseases · 2017Article
- LAMPhimerus: A novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples.PLoS neglected tropical diseases · 2017Article
- Molecular characterization of the unique Mesostephanus appendiculatus (Trematoda: Cyathocotylidae) by small ribosomal RNA from Egypt.Parasitology research · 2017Article
- Development of a PCR Assay for Diagnosing Trematode (Opisthorchis and Haplorchis) Infections in Human Stools.The American journal of tropical medicine and hygiene · 2017Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Opisthorchis viverrini and Clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in Asia. The standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. However, the method has low sensitivity, and eggs of O. viverrini and C. sinensis are difficult to distinguish from each other and from those of some other trematodes. Here, we report a multiplex real-time PCR coupled with high resolution melting (HRM) analysis for the differentiation of O. viverrini and C. sinensis eggs in fecal samples. Using 2 pairs of species-specific primers, DNA sequences from a portion of the mitochondrial NADH dehydrogenase subunit 2 (nad 2) gene, were amplified to generate 209 and 165 bp products for O. viverrini and C. sinensis, respectively. The distinct characteristics of HRM patterns were analyzed, and the melting temperatures peaked at 82.4±0.09℃ and 85.9±0.08℃ for O. viverrini and C. sinensis, respectively. This technique was able to detect as few as 1 egg of O. viverrini and 2 eggs of C. sinensis in a 150 mg fecal sample, which is equivalent to 7 and 14 eggs per gram of feces, respectively. The method is species-specific, rapid, simple, and does not require fluorescent probes or post-PCR processing for discrimination of eggs of the 2 species. It offers a new tool for differentiation and detection of Asian liver fluke infections in stool specimens.
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