ArticleThe Korean journal of parasitology2013
Rapid detection and identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in mosquito vectors and blood samples by high resolution melting real-time PCR.
Article in The Korean journal of parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- DetectingPathogens (Basel, Switzerland) · 2024Review
- Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis.Pathogens (Basel, Switzerland) · 2024Review
- Assessing Phylogeographic Patterns and Genetic Diversity inZoological studies · 2024Article
- Mosquitoes, Lymphatic Filariasis, and Public Health: A Systematic Review ofPathogens (Basel, Switzerland) · 2023Review
- Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting).Acta parasitologica · 2022Article
- Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases.Clinical microbiology reviews · 2021Article
- The Incidence ofPathogens (Basel, Switzerland) · 2021Article
- The use of molecular xenomonitoring for surveillance of mosquito-borne diseases.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction.Parasitology research · 2016Article
- Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions?Parasitology research · 2016Article
- Trypanosome infection rates in tsetse flies in the "silent" sleeping sickness focus of Bafia in the Centre Region in Cameroon.Parasites & vectors · 2015Article
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Authors and funding
11 authors.
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Abstract
A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.
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